Quantification of astrocytic synaptic pruning in mouse hippocampal slices in response to ex vivo Aβ treatment via colocalization analysis with C1q

Arpit Kumar Pradhan; Qinfang Shi; Katharina Johanna Tartler; Gerhard Rammes

doi:10.1016/j.xpro.2022.101687

Quantification of astrocytic synaptic pruning in mouse hippocampal slices in response to ex vivo Aβ treatment via colocalization analysis with C1q

Arpit Kumar Pradhan, Qinfang Shi, Katharina Johanna Tartler, Gerhard Rammes

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Quantification of synaptic engulfment is an indirect measurement of synaptic pruning. Here, we provide a detailed protocol for the volumetric rendering of individual high-resolution astrocytes in the CA1 region of hippocampus in an ex vivo model of amyloid-beta (Aβ) treatment. The protocol includes the treatment of free-floating sections with Aβ peptide and confocal imaging of individual astrocytes. We also provide a comprehensive analysis for 3D rendering of astrocytes and assessment of synaptic engulfment via “eat-me tag” C1q protein.

Original language	English
Article number	101687
Journal	STAR Protocols
Volume	3
Issue number	4
DOIs	https://doi.org/10.1016/j.xpro.2022.101687
State	Published - 16 Dec 2022
Externally published	Yes

Keywords

Cell biology
Immunology
Microscopy
Neuroscience

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10.1016/j.xpro.2022.101687

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title = "Quantification of astrocytic synaptic pruning in mouse hippocampal slices in response to ex vivo Aβ treatment via colocalization analysis with C1q",

abstract = "Quantification of synaptic engulfment is an indirect measurement of synaptic pruning. Here, we provide a detailed protocol for the volumetric rendering of individual high-resolution astrocytes in the CA1 region of hippocampus in an ex vivo model of amyloid-beta (Aβ) treatment. The protocol includes the treatment of free-floating sections with Aβ peptide and confocal imaging of individual astrocytes. We also provide a comprehensive analysis for 3D rendering of astrocytes and assessment of synaptic engulfment via “eat-me tag” C1q protein.",

keywords = "Cell biology, Immunology, Microscopy, Neuroscience",

author = "Pradhan, {Arpit Kumar} and Qinfang Shi and Tartler, {Katharina Johanna} and Gerhard Rammes",

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year = "2022",

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AU - Pradhan, Arpit Kumar

AU - Shi, Qinfang

AU - Tartler, Katharina Johanna

AU - Rammes, Gerhard

PY - 2022/12/16

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N2 - Quantification of synaptic engulfment is an indirect measurement of synaptic pruning. Here, we provide a detailed protocol for the volumetric rendering of individual high-resolution astrocytes in the CA1 region of hippocampus in an ex vivo model of amyloid-beta (Aβ) treatment. The protocol includes the treatment of free-floating sections with Aβ peptide and confocal imaging of individual astrocytes. We also provide a comprehensive analysis for 3D rendering of astrocytes and assessment of synaptic engulfment via “eat-me tag” C1q protein.

AB - Quantification of synaptic engulfment is an indirect measurement of synaptic pruning. Here, we provide a detailed protocol for the volumetric rendering of individual high-resolution astrocytes in the CA1 region of hippocampus in an ex vivo model of amyloid-beta (Aβ) treatment. The protocol includes the treatment of free-floating sections with Aβ peptide and confocal imaging of individual astrocytes. We also provide a comprehensive analysis for 3D rendering of astrocytes and assessment of synaptic engulfment via “eat-me tag” C1q protein.

KW - Cell biology

KW - Immunology

KW - Microscopy

KW - Neuroscience

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ER -

Quantification of astrocytic synaptic pruning in mouse hippocampal slices in response to ex vivo Aβ treatment via colocalization analysis with C1q

Abstract

Keywords

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