QTL mapping for resistance to fire blight using several erwinia amylovora strains resulting in different host-pathogen interactions

Th Wöhner; I. Vogt; A. Peil; H. Flachowsky; M. V. Hanke; C. Gessler; G. A.L. Broggini; J. Fahrentrapp; K. Richter; T. Garcia-Libreros

doi:10.17660/ActaHortic.2013.976.72

QTL mapping for resistance to fire blight using several erwinia amylovora strains resulting in different host-pathogen interactions

Th Wöhner, I. Vogt, A. Peil, H. Flachowsky, M. V. Hanke, C. Gessler, G. A.L. Broggini, J. Fahrentrapp, K. Richter, T. Garcia-Libreros

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1 Scopus citations

Abstract

QTL mapping of fire blight resistance in apple is an effective tool to determine associations between regions in the genome of apple and resistance to the bacterial disease. Several wild Malus accessions of different apple species have been identified as resistant to fire blight making them valuable as donors for the introgression of resistance to the cultivated apple Malus × domestica Borkh. Resistant accessions of the wild species M. baccata, M. fusca and M. × robusta 5 (Mr5) were inoculated with the wild type strain Ea1189 and the AvrRpt2EA deletion mutant (pZYRKD3-1). While M. baccata and M. fusca showed no symptoms to pZYRKD3-1, the resistance of M. × robusta 5 was overcome by pZYRKD3-1 with an average necrosis length of 52% respectively. Inoculation of the mapping population 'Idared' × Mr5 with the strain Ea 1189 results in the confirmation of the QTL on LG 3 in Mr5; this QTL completely broke down after inoculation with pZYRKD3-1, but two minor QTLs on LG 7 and LG 11 were detected.

Original language	English
Pages (from-to)	509-512
Number of pages	4
Journal	Acta Horticulturae
Volume	976
DOIs	https://doi.org/10.17660/ActaHortic.2013.976.72
State	Published - 28 Feb 2013
Externally published	Yes

Keywords

AvrRpt2Ea
Deletion mutant strain
Interval mapping

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10.17660/ActaHortic.2013.976.72

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Wöhner, T., Vogt, I., Peil, A., Flachowsky, H., Hanke, M. V., Gessler, C., Broggini, G. A. L., Fahrentrapp, J., Richter, K., & Garcia-Libreros, T. (2013). QTL mapping for resistance to fire blight using several erwinia amylovora strains resulting in different host-pathogen interactions. Acta Horticulturae, 976, 509-512. https://doi.org/10.17660/ActaHortic.2013.976.72

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title = "QTL mapping for resistance to fire blight using several erwinia amylovora strains resulting in different host-pathogen interactions",

abstract = "QTL mapping of fire blight resistance in apple is an effective tool to determine associations between regions in the genome of apple and resistance to the bacterial disease. Several wild Malus accessions of different apple species have been identified as resistant to fire blight making them valuable as donors for the introgression of resistance to the cultivated apple Malus × domestica Borkh. Resistant accessions of the wild species M. baccata, M. fusca and M. × robusta 5 (Mr5) were inoculated with the wild type strain Ea1189 and the AvrRpt2EA deletion mutant (pZYRKD3-1). While M. baccata and M. fusca showed no symptoms to pZYRKD3-1, the resistance of M. × robusta 5 was overcome by pZYRKD3-1 with an average necrosis length of 52% respectively. Inoculation of the mapping population 'Idared' × Mr5 with the strain Ea 1189 results in the confirmation of the QTL on LG 3 in Mr5; this QTL completely broke down after inoculation with pZYRKD3-1, but two minor QTLs on LG 7 and LG 11 were detected.",

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author = "Th W{\"o}hner and I. Vogt and A. Peil and H. Flachowsky and Hanke, {M. V.} and C. Gessler and Broggini, {G. A.L.} and J. Fahrentrapp and K. Richter and T. Garcia-Libreros",

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doi = "10.17660/ActaHortic.2013.976.72",

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AU - Vogt, I.

AU - Peil, A.

AU - Flachowsky, H.

AU - Hanke, M. V.

AU - Gessler, C.

AU - Broggini, G. A.L.

AU - Fahrentrapp, J.

AU - Richter, K.

AU - Garcia-Libreros, T.

PY - 2013/2/28

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N2 - QTL mapping of fire blight resistance in apple is an effective tool to determine associations between regions in the genome of apple and resistance to the bacterial disease. Several wild Malus accessions of different apple species have been identified as resistant to fire blight making them valuable as donors for the introgression of resistance to the cultivated apple Malus × domestica Borkh. Resistant accessions of the wild species M. baccata, M. fusca and M. × robusta 5 (Mr5) were inoculated with the wild type strain Ea1189 and the AvrRpt2EA deletion mutant (pZYRKD3-1). While M. baccata and M. fusca showed no symptoms to pZYRKD3-1, the resistance of M. × robusta 5 was overcome by pZYRKD3-1 with an average necrosis length of 52% respectively. Inoculation of the mapping population 'Idared' × Mr5 with the strain Ea 1189 results in the confirmation of the QTL on LG 3 in Mr5; this QTL completely broke down after inoculation with pZYRKD3-1, but two minor QTLs on LG 7 and LG 11 were detected.

AB - QTL mapping of fire blight resistance in apple is an effective tool to determine associations between regions in the genome of apple and resistance to the bacterial disease. Several wild Malus accessions of different apple species have been identified as resistant to fire blight making them valuable as donors for the introgression of resistance to the cultivated apple Malus × domestica Borkh. Resistant accessions of the wild species M. baccata, M. fusca and M. × robusta 5 (Mr5) were inoculated with the wild type strain Ea1189 and the AvrRpt2EA deletion mutant (pZYRKD3-1). While M. baccata and M. fusca showed no symptoms to pZYRKD3-1, the resistance of M. × robusta 5 was overcome by pZYRKD3-1 with an average necrosis length of 52% respectively. Inoculation of the mapping population 'Idared' × Mr5 with the strain Ea 1189 results in the confirmation of the QTL on LG 3 in Mr5; this QTL completely broke down after inoculation with pZYRKD3-1, but two minor QTLs on LG 7 and LG 11 were detected.

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KW - Deletion mutant strain

KW - Interval mapping

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QTL mapping for resistance to fire blight using several erwinia amylovora strains resulting in different host-pathogen interactions

Abstract

Keywords

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