QTL analysis for ascorbic acid content in strawberry fruit reveals a complex genetic architecture and association with GDP-L-galactose phosphorylase

Pilar Muñoz, Cristina Castillejo, José Antonio Gómez, Luis Miranda, Silke Lesemann, Klaus Olbricht, Aurélie Petit, Philippe Chartier, Annika Haugeneder, Johanna Trinkl, Luca Mazzoni, Agnieszka Masny, Edward Zurawicz, Freya Maria Rosemarie Ziegler, Björn Usadel, Wilfried Schwab, Béatrice Denoyes, Bruno Mezzetti, Sonia Osorio, José F. Sánchez-SevillaIraida Amaya

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Strawberry (Fragaria × ananassa) fruits are an excellent source of L-ascorbic acid (AsA), a powerful antioxidant for plants and humans. Identifying the genetic components underlying AsA accumulation is crucial for enhancing strawberry nutritional quality. Here, we unravel the genetic architecture of AsA accumulation using an F1 population derived from parental lines 'Candonga' and 'Senga Sengana', adapted to distinct Southern and Northern European areas. To account for environmental effects, the F1 and parental lines were grown and phenotyped in five locations across Europe (France, Germany, Italy, Poland and Spain). Fruit AsA content displayed normal distribution typical of quantitative traits and ranged five-fold, with significant differences among genotypes and environments. AsA content in each country and the average in all of them was used in combination with 6,974 markers for quantitative trait locus (QTL) analysis. Environmentally stable QTLs for AsA content were detected in linkage group (LG) 3A, LG 5A, LG 5B, LG 6B and LG 7C. Candidate genes were identified within stable QTL intervals and expression analysis in lines with contrasting AsA content suggested that GDP-L-Galactose Phosphorylase FaGGP(3A), and the chloroplast-located AsA transporter gene FaPHT4;4(7C) might be the underlying genetic factors for QTLs on LG 3A and 7C, respectively. We show that recessive alleles of FaGGP(3A) inherited from both parental lines increase fruit AsA content. Furthermore, expression of FaGGP(3A) was two-fold higher in lines with high AsA. Markers here identified represent a useful resource for efficient selection of new strawberry cultivars with increased AsA content.

Original languageEnglish
Article numberuhad006
JournalHorticulture Research
Volume10
Issue number3
DOIs
StatePublished - 2023

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