qPCR for quantitative validation of metagenomic data

Stefanie Schulz; Fabian Bergkemper; Maria De Vries; Anne Schöler; Michael Schloter

doi:10.1007/s12268-016-0684-1

qPCR for quantitative validation of metagenomic data

Stefanie Schulz, Fabian Bergkemper, Maria De Vries, Anne Schöler, Michael Schloter

Helmholtz Zentrum München German Research Center for Environmental Health

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Microorganisms are essential to maintain ecosystem functions and health as they catalyze most keystone processes. Formerly the quantification of key functions was performed by broad ranged primers, which were mainly based on genomes of cultivated microbes. To take advantage of the enormous development of next generation sequencing technologies, we propose to use metagenomic data for the design of new primer systems. Here we describe a two-phasic approach for a targeted and site specific primer design for qPCR.

Translated title of the contribution	qPCR zur quantitativen Validierung von Metagenomdaten
Original language	English
Pages (from-to)	265-269
Number of pages	5
Journal	BioSpektrum
Volume	22
Issue number	3
DOIs	https://doi.org/10.1007/s12268-016-0684-1
State	Published - May 2016
Externally published	Yes

Access to Document

10.1007/s12268-016-0684-1

Cite this

@article{e871cbc597b140659f2d7d67b7d30d50,

title = "qPCR for quantitative validation of metagenomic data",

abstract = "Microorganisms are essential to maintain ecosystem functions and health as they catalyze most keystone processes. Formerly the quantification of key functions was performed by broad ranged primers, which were mainly based on genomes of cultivated microbes. To take advantage of the enormous development of next generation sequencing technologies, we propose to use metagenomic data for the design of new primer systems. Here we describe a two-phasic approach for a targeted and site specific primer design for qPCR.",

author = "Stefanie Schulz and Fabian Bergkemper and {De Vries}, Maria and Anne Sch{\"o}ler and Michael Schloter",

note = "Publisher Copyright: {\textcopyright} 2016, Springer-Verlag Berlin Heidelberg.",

year = "2016",

month = may,

doi = "10.1007/s12268-016-0684-1",

language = "English",

volume = "22",

pages = "265--269",

journal = "BioSpektrum",

issn = "0947-0867",

publisher = "Springer Spektrum",

number = "3",

}

TY - JOUR

T1 - qPCR for quantitative validation of metagenomic data

AU - Schulz, Stefanie

AU - Bergkemper, Fabian

AU - De Vries, Maria

AU - Schöler, Anne

AU - Schloter, Michael

PY - 2016/5

Y1 - 2016/5

N2 - Microorganisms are essential to maintain ecosystem functions and health as they catalyze most keystone processes. Formerly the quantification of key functions was performed by broad ranged primers, which were mainly based on genomes of cultivated microbes. To take advantage of the enormous development of next generation sequencing technologies, we propose to use metagenomic data for the design of new primer systems. Here we describe a two-phasic approach for a targeted and site specific primer design for qPCR.

AB - Microorganisms are essential to maintain ecosystem functions and health as they catalyze most keystone processes. Formerly the quantification of key functions was performed by broad ranged primers, which were mainly based on genomes of cultivated microbes. To take advantage of the enormous development of next generation sequencing technologies, we propose to use metagenomic data for the design of new primer systems. Here we describe a two-phasic approach for a targeted and site specific primer design for qPCR.

UR - http://www.scopus.com/inward/record.url?scp=84964778044&partnerID=8YFLogxK

U2 - 10.1007/s12268-016-0684-1

DO - 10.1007/s12268-016-0684-1

M3 - Article

AN - SCOPUS:84964778044

SN - 0947-0867

VL - 22

SP - 265

EP - 269

JO - BioSpektrum

JF - BioSpektrum

IS - 3

ER -

qPCR for quantitative validation of metagenomic data

Abstract

Access to Document

Other files and links

Fingerprint

Cite this