qPCR for quantitative validation of metagenomic data

Stefanie Schulz, Fabian Bergkemper, Maria De Vries, Anne Schöler, Michael Schloter

Research output: Contribution to journalArticlepeer-review

1 Scopus citations


Microorganisms are essential to maintain ecosystem functions and health as they catalyze most keystone processes. Formerly the quantification of key functions was performed by broad ranged primers, which were mainly based on genomes of cultivated microbes. To take advantage of the enormous development of next generation sequencing technologies, we propose to use metagenomic data for the design of new primer systems. Here we describe a two-phasic approach for a targeted and site specific primer design for qPCR.

Translated title of the contributionqPCR zur quantitativen Validierung von Metagenomdaten
Original languageEnglish
Pages (from-to)265-269
Number of pages5
Issue number3
StatePublished - May 2016
Externally publishedYes


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