Psychroflexus halocasei sp. nov., isolated from a microbial consortium on a cheese

Herbert Seiler, Anne Bleicher, Hans Jürgen Busse, Josef Hüner, Siegfried Scherer

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A novel halophilic, Gram-reaction-negative, strictly aerobic, non-motile, rod-shaped and oxidaseand catalase-positive bacterial strain, designated WCC 4520T, was isolated from a semi-hard, Raclette-type cheese. The colonies were yellow-orange; flexirubin-type pigments were not found. The strain hydrolysed gelatin, hippurate, tyrosine and Tweens 20 and 80. Optimal growth was observed with 6-8% (w/v) NaCl, at pH 7-8 and at 27-30 °C. The genomic DNA G+C content was 33.6 mol%. In phylogenetic analysis based on 16S rRNA gene sequences, strain WCC 4520T appeared to be a member of the family Flavobacteriaceae and the closest phylogenetic neighbours were identified as Psychroflexus gondwanensis DSM 5423T (94.0% 16S rRNA gene sequence similarity) and Psychroflexus salinarum CCUG 56752T (94.0 %). The predominant cellular fatty acids were iso-C15: 0, anteiso-C15: 0, iso-C15: 1 G and iso-C17: 0 3-OH. The only detected quinone was MK-6 and the major polar lipids were phosphatidylethanolamine, an unidentified aminolipid and an unidentified polar lipid. Minor polar lipids and traces of polyamines were also detected. On the basis of the data presented, strain WCC 4520T represents a novel species of the genus Psychroflexus, for which the name Psychroflexus halocasei sp. nov. is proposed. The type strain is WCC 4520T (=LMG 2585=T5CCUG 59705T).

Original languageEnglish
Pages (from-to)1850-1856
Number of pages7
JournalInternational Journal of Systematic and Evolutionary Microbiology
Issue number8
StatePublished - Aug 2012


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