Protoplast fusion in banana (Musa spp.): Comparison of chemical (PEG: Polyethylene glycol) and electrical procedure

Akym Assani, Djamila Chabane, Robert Haïcour, Frédéric Bakry, Gerhard Wenzel, Bärbel Foroughi-Wehr

Research output: Contribution to journalArticlepeer-review

31 Scopus citations

Abstract

Optimization of protoplast fusion parameters is a prerequisite for the establishment of somatic fusion technology for banana breeding. In the present investigations, we compared the most frequently used fusion methods: the electrofusion technique and chemical procedure (polyethylene glycol). With regard to frequency of binary fusion, protoplast fusion with the fusogen polyethylene glycol was best. Conversely, electric fusion was found to be better with respect to mitotic activities, somatic embryogenesis and plantlet regeneration rate.

Original languageEnglish
Pages (from-to)145-151
Number of pages7
JournalPlant Cell, Tissue and Organ Culture
Volume83
Issue number2
DOIs
StatePublished - Nov 2005
Externally publishedYes

Keywords

  • Electrofusion
  • Musa spp.
  • Polyethylene glycol
  • Protoplast fusion

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