Protocol for assessing and visualizing cell microaggregate formation in whole blood by imaging flow cytometry

Johannes Krell; Komal Kumar Javarappa; Angie Wenedy; Andrew L. Frelinger; Laurent Renia; Clarissa Prazeres da Costa; Martin Schlegel; Percy Knolle; Gerhard Schneider; Oliver Hayden

doi:10.1016/j.xpro.2025.103598

Protocol for assessing and visualizing cell microaggregate formation in whole blood by imaging flow cytometry

Johannes Krell, Komal Kumar Javarappa, Angie Wenedy, Andrew L. Frelinger, Laurent Renia, Clarissa Prazeres da Costa, Martin Schlegel, Percy Knolle, Gerhard Schneider, Oliver Hayden

Research output: Contribution to journal › Article › peer-review

Abstract

Blood cell aggregates are clinically useful biomarkers in a number of medical disorders. This protocol provides accurate and quantitative analysis of cell aggregates using a small volume of whole blood and imaging flow cytometry. We describe steps for sample collection, staining, and measurement. We then detail gating procedures and analysis of cell morphology. Sample preparation artifacts, activation, and morphological changes of cells are mitigated by omitting erythrocyte lysis and leukocyte isolation while maintaining high-throughput accurate imaging of leukocytes and platelets.

Original language	English
Article number	103598
Journal	STAR Protocols
Volume	6
Issue number	1
DOIs	https://doi.org/10.1016/j.xpro.2025.103598
State	Published - 21 Mar 2025

Keywords

Clinical Protocol
Flow Cytometry
Immunology
Microscopy

Access to Document

10.1016/j.xpro.2025.103598

Cite this

@article{3307adb54fce4975bc089520bdee714f,

title = "Protocol for assessing and visualizing cell microaggregate formation in whole blood by imaging flow cytometry",

abstract = "Blood cell aggregates are clinically useful biomarkers in a number of medical disorders. This protocol provides accurate and quantitative analysis of cell aggregates using a small volume of whole blood and imaging flow cytometry. We describe steps for sample collection, staining, and measurement. We then detail gating procedures and analysis of cell morphology. Sample preparation artifacts, activation, and morphological changes of cells are mitigated by omitting erythrocyte lysis and leukocyte isolation while maintaining high-throughput accurate imaging of leukocytes and platelets.",

keywords = "Clinical Protocol, Flow Cytometry, Immunology, Microscopy",

author = "Johannes Krell and Javarappa, {Komal Kumar} and Angie Wenedy and Frelinger, {Andrew L.} and Laurent Renia and {Prazeres da Costa}, Clarissa and Martin Schlegel and Percy Knolle and Gerhard Schneider and Oliver Hayden",

note = "Publisher Copyright: {\textcopyright} 2025 The Authors",

year = "2025",

month = mar,

day = "21",

doi = "10.1016/j.xpro.2025.103598",

language = "English",

volume = "6",

journal = "STAR Protocols",

issn = "2666-1667",

publisher = "Cell Press",

number = "1",

}

TY - JOUR

T1 - Protocol for assessing and visualizing cell microaggregate formation in whole blood by imaging flow cytometry

AU - Krell, Johannes

AU - Javarappa, Komal Kumar

AU - Wenedy, Angie

AU - Frelinger, Andrew L.

AU - Renia, Laurent

AU - Prazeres da Costa, Clarissa

AU - Schlegel, Martin

AU - Knolle, Percy

AU - Schneider, Gerhard

AU - Hayden, Oliver

PY - 2025/3/21

Y1 - 2025/3/21

N2 - Blood cell aggregates are clinically useful biomarkers in a number of medical disorders. This protocol provides accurate and quantitative analysis of cell aggregates using a small volume of whole blood and imaging flow cytometry. We describe steps for sample collection, staining, and measurement. We then detail gating procedures and analysis of cell morphology. Sample preparation artifacts, activation, and morphological changes of cells are mitigated by omitting erythrocyte lysis and leukocyte isolation while maintaining high-throughput accurate imaging of leukocytes and platelets.

AB - Blood cell aggregates are clinically useful biomarkers in a number of medical disorders. This protocol provides accurate and quantitative analysis of cell aggregates using a small volume of whole blood and imaging flow cytometry. We describe steps for sample collection, staining, and measurement. We then detail gating procedures and analysis of cell morphology. Sample preparation artifacts, activation, and morphological changes of cells are mitigated by omitting erythrocyte lysis and leukocyte isolation while maintaining high-throughput accurate imaging of leukocytes and platelets.

KW - Clinical Protocol

KW - Flow Cytometry

KW - Immunology

KW - Microscopy

UR - http://www.scopus.com/inward/record.url?scp=85215865289&partnerID=8YFLogxK

U2 - 10.1016/j.xpro.2025.103598

DO - 10.1016/j.xpro.2025.103598

M3 - Article

AN - SCOPUS:85215865289

SN - 2666-1667

VL - 6

JO - STAR Protocols

JF - STAR Protocols

IS - 1

M1 - 103598

ER -

Protocol for assessing and visualizing cell microaggregate formation in whole blood by imaging flow cytometry

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this