Abstract
In this work we examined the effect of non-thermal low electric treatment on the protein expression of Saccharomyces cerevisiae. The electric field was produced from therapeutic device Powertube QuickZap, which is intended for the electronic stimulation of nerves. It is used for selftreatment of symptoms and pain. Previous experiments have shown that low frequency electric or magnetic fields cause changes in protein induction. The present study confirms these observations. Two-dimensional gel electrophoresis (2-DE), Progenesis SameSpots software and matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF-MS) were used to examine changes in protein expression. In response to 3 hours nonthermal low electric field exposure, the enzyme homoserin dehydrogenase was overexpressed. This result suggests that this enzyme may play an important role in the adaptation of Saccharomyces cerevisiae to electric stress. The role of an electric field as a co-stressing factor is discussed.
| Original language | English |
|---|---|
| Pages (from-to) | 109-113 |
| Number of pages | 5 |
| Journal | Fresenius Environmental Bulletin |
| Volume | 20 |
| Issue number | 1 |
| State | Published - 2011 |
Keywords
- Matrix assisted laser desorption ionlzation-time of flight mass spectrometry
- Non-thermal low electric field
- Stress response
- Two-dimensional gel electrophoresis