Proteomic study on the cellular responses to non-thermal low electric field in saccharomyces cerevisiae

Ali Fanous, Perihan Ekici, Angelika Görg, Harun Parlar

Research output: Contribution to journalArticlepeer-review

Abstract

In this work we examined the effect of non-thermal low electric treatment on the protein expression of Saccharomyces cerevisiae. The electric field was produced from therapeutic device Powertube QuickZap, which is intended for the electronic stimulation of nerves. It is used for selftreatment of symptoms and pain. Previous experiments have shown that low frequency electric or magnetic fields cause changes in protein induction. The present study confirms these observations. Two-dimensional gel electrophoresis (2-DE), Progenesis SameSpots software and matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF-MS) were used to examine changes in protein expression. In response to 3 hours nonthermal low electric field exposure, the enzyme homoserin dehydrogenase was overexpressed. This result suggests that this enzyme may play an important role in the adaptation of Saccharomyces cerevisiae to electric stress. The role of an electric field as a co-stressing factor is discussed.

Original languageEnglish
Pages (from-to)109-113
Number of pages5
JournalFresenius Environmental Bulletin
Volume20
Issue number1
StatePublished - 2011

Keywords

  • Matrix assisted laser desorption ionlzation-time of flight mass spectrometry
  • Non-thermal low electric field
  • Stress response
  • Two-dimensional gel electrophoresis

Fingerprint

Dive into the research topics of 'Proteomic study on the cellular responses to non-thermal low electric field in saccharomyces cerevisiae'. Together they form a unique fingerprint.

Cite this