Proteome labeling and protein identification in specific tissues and at specific developmental stages in an animal

Thomas S. Elliott, Fiona M. Townsley, Ambra Bianco, Russell J. Ernst, Amit Sachdeva, Simon J. Elsässer, Lloyd Davis, Kathrin Lang, Rudolf Pisa, Sebastian Greiss, Kathryn S. Lilley, Jason W. Chin

Research output: Contribution to journalArticlepeer-review

145 Scopus citations

Abstract

Identifying the proteins synthesized at specific times in cells of interest in an animal will facilitate the study of cellular functions and dynamic processes. Here we introduce stochastic orthogonal recoding of translation with chemoselective modification (SORT-M) to address this challenge. SORT-M involves modifying cells to express an orthogonal aminoacyl-tRNA synthetase/tRNA pair to enable the incorporation of chemically modifiable analogs of amino acids at diverse sense codons in cells in rich media. We apply SORT-M to Drosophila melanogaster fed standard food to label and image proteins in specific tissues at precise developmental stages with diverse chemistries, including cyclopropene-tetrazine inverse electron demand Diels-Alder cycloaddition reactions. We also use SORT-M to identify proteins synthesized in germ cells of the fly ovary without dissection. SORT-M will facilitate the definition of proteins synthesized in specific sets of cells to study development, and learning and memory in flies, and may be extended to other animals.

Original languageEnglish
Pages (from-to)465-472
Number of pages8
JournalNature Biotechnology
Volume32
Issue number5
DOIs
StatePublished - May 2014
Externally publishedYes

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