Protein-RNA interfaces probed by 1H-detected MAS solid-state NMR spectroscopy

Sam Asami; Magdalena Rakwalska-Bange; Teresa Carlomagno; Bernd Reif

doi:10.1002/anie.201208024

Protein-RNA interfaces probed by ¹H-detected MAS solid-state NMR spectroscopy

Sam Asami
, Magdalena Rakwalska-Bange
, Teresa Carlomagno
, Bernd Reif

Associate Professorship of Solid-State NMR spectroscopy

Technical University of Munich
Helmholtz Zentrum München German Research Center for Environmental Health
European Molecular Biology Laboratory Heidelberg

Research output: Contribution to journal › Article › peer-review

52 Scopus citations

Abstract

Both protonated and deuterated samples were employed in the study of the L7Ae box C/D RNA complex by ¹H-detected solid-state NMR spectroscopy. This approach yielded high-resolution spectra and was used to determine the intermolecular interface and extract structural parameters with high accuracy.

Original language	English
Pages (from-to)	2345-2349
Number of pages	5
Journal	Angewandte Chemie International Edition in English
Volume	52
Issue number	8
DOIs	https://doi.org/10.1002/anie.201208024
State	Published - 18 Feb 2013

Keywords

NMR spectroscopy
RNA
proteins
proton detection
structural restraints

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10.1002/anie.201208024

Cite this

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title = "Protein-RNA interfaces probed by 1H-detected MAS solid-state NMR spectroscopy",

abstract = "Both protonated and deuterated samples were employed in the study of the L7Ae box C/D RNA complex by 1H-detected solid-state NMR spectroscopy. This approach yielded high-resolution spectra and was used to determine the intermolecular interface and extract structural parameters with high accuracy.",

keywords = "NMR spectroscopy, RNA, proteins, proton detection, structural restraints",

author = "Sam Asami and Magdalena Rakwalska-Bange and Teresa Carlomagno and Bernd Reif",

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T1 - Protein-RNA interfaces probed by 1H-detected MAS solid-state NMR spectroscopy

AU - Asami, Sam

AU - Rakwalska-Bange, Magdalena

AU - Carlomagno, Teresa

AU - Reif, Bernd

PY - 2013/2/18

Y1 - 2013/2/18

N2 - Both protonated and deuterated samples were employed in the study of the L7Ae box C/D RNA complex by 1H-detected solid-state NMR spectroscopy. This approach yielded high-resolution spectra and was used to determine the intermolecular interface and extract structural parameters with high accuracy.

AB - Both protonated and deuterated samples were employed in the study of the L7Ae box C/D RNA complex by 1H-detected solid-state NMR spectroscopy. This approach yielded high-resolution spectra and was used to determine the intermolecular interface and extract structural parameters with high accuracy.

KW - NMR spectroscopy

KW - RNA

KW - proteins

KW - proton detection

KW - structural restraints

UR - https://www.scopus.com/pages/publications/84873932296

U2 - 10.1002/anie.201208024

DO - 10.1002/anie.201208024

M3 - Article

C2 - 23335059

AN - SCOPUS:84873932296

SN - 1433-7851

VL - 52

SP - 2345

EP - 2349

JO - Angewandte Chemie International Edition in English

JF - Angewandte Chemie International Edition in English

IS - 8

ER -

Protein-RNA interfaces probed by ¹H-detected MAS solid-state NMR spectroscopy

Abstract

Keywords

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