Protein-modified nanocrystalline diamond thin films for biosensor applications

Andreas Härtl; Evelyn Schmich; Jose A. Garrido; Jorge Hernando; Silvia C.R. Catharino; Stefan Walter; Peter Feulner; Alexander Kromka; Doris Steinmüller; Martin Stutzmann

doi:10.1038/nmat1204

Protein-modified nanocrystalline diamond thin films for biosensor applications

Andreas Härtl, Evelyn Schmich, Jose A. Garrido, Jorge Hernando, Silvia C.R. Catharino, Stefan Walter, Peter Feulner, Alexander Kromka, Doris Steinmüller, Martin Stutzmann

Research output: Contribution to journal › Article › peer-review

502 Scopus citations

Abstract

The possibility of proteins being attached covalently to nanocrystalline diamond thin films was shown. Using a photochemical process to generate a surface layer of amino groups, to which proteins were covalently attached, hydrogen-terminated nanocrystalline diamond films were modified. A direct electron transfer between the enzyme's redox center and the diamond electrode was detected, after the functionalization of nanocrystalline diamond electrodes with the enzyme catalase. It was suggested that the dual role of nanocrystalline diamond, as a substrate for biofunctionalization and as an electrode, makes it a promising candidate for future biosensor applications.

Original language	English
Pages (from-to)	736-742
Number of pages	7
Journal	Nature Materials
Volume	3
Issue number	10
DOIs	https://doi.org/10.1038/nmat1204
State	Published - Oct 2004

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title = "Protein-modified nanocrystalline diamond thin films for biosensor applications",

abstract = "The possibility of proteins being attached covalently to nanocrystalline diamond thin films was shown. Using a photochemical process to generate a surface layer of amino groups, to which proteins were covalently attached, hydrogen-terminated nanocrystalline diamond films were modified. A direct electron transfer between the enzyme's redox center and the diamond electrode was detected, after the functionalization of nanocrystalline diamond electrodes with the enzyme catalase. It was suggested that the dual role of nanocrystalline diamond, as a substrate for biofunctionalization and as an electrode, makes it a promising candidate for future biosensor applications.",

author = "Andreas H{\"a}rtl and Evelyn Schmich and Garrido, {Jose A.} and Jorge Hernando and Catharino, {Silvia C.R.} and Stefan Walter and Peter Feulner and Alexander Kromka and Doris Steinm{\"u}ller and Martin Stutzmann",

year = "2004",

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T1 - Protein-modified nanocrystalline diamond thin films for biosensor applications

AU - Härtl, Andreas

AU - Schmich, Evelyn

AU - Garrido, Jose A.

AU - Hernando, Jorge

AU - Catharino, Silvia C.R.

AU - Walter, Stefan

AU - Feulner, Peter

AU - Kromka, Alexander

AU - Steinmüller, Doris

AU - Stutzmann, Martin

PY - 2004/10

Y1 - 2004/10

N2 - The possibility of proteins being attached covalently to nanocrystalline diamond thin films was shown. Using a photochemical process to generate a surface layer of amino groups, to which proteins were covalently attached, hydrogen-terminated nanocrystalline diamond films were modified. A direct electron transfer between the enzyme's redox center and the diamond electrode was detected, after the functionalization of nanocrystalline diamond electrodes with the enzyme catalase. It was suggested that the dual role of nanocrystalline diamond, as a substrate for biofunctionalization and as an electrode, makes it a promising candidate for future biosensor applications.

AB - The possibility of proteins being attached covalently to nanocrystalline diamond thin films was shown. Using a photochemical process to generate a surface layer of amino groups, to which proteins were covalently attached, hydrogen-terminated nanocrystalline diamond films were modified. A direct electron transfer between the enzyme's redox center and the diamond electrode was detected, after the functionalization of nanocrystalline diamond electrodes with the enzyme catalase. It was suggested that the dual role of nanocrystalline diamond, as a substrate for biofunctionalization and as an electrode, makes it a promising candidate for future biosensor applications.

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U2 - 10.1038/nmat1204

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VL - 3

SP - 736

EP - 742

JO - Nature Materials

JF - Nature Materials

IS - 10

ER -

Protein-modified nanocrystalline diamond thin films for biosensor applications

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