Protection and Reactivation of the [NiFeSe] Hydrogenase from Desulfovibrio vulgaris Hildenborough under Oxidative Conditions

Adrian Ruff; Julian Szczesny; Sónia Zacarias; Inês A.C. Pereira; Nicolas Plumeré; Wolfgang Schuhmann

doi:10.1021/acsenergylett.7b00167

Protection and Reactivation of the [NiFeSe] Hydrogenase from Desulfovibrio vulgaris Hildenborough under Oxidative Conditions

Adrian Ruff
, Julian Szczesny
, Sónia Zacarias
, Inês A.C. Pereira
, Nicolas Plumeré
, Wolfgang Schuhmann

Research output: Contribution to journal › Article › peer-review

47 Scopus citations

Abstract

We report on the fabrication of bioanodes for H₂ oxidation based on [NiFeSe] hydrogenase. The enzyme was electrically wired by means of a specifically designed low-potential viologen-modified polymer, which delivers benchmark H₂ oxidizing currents even under deactivating conditions owing to efficient protection against O₂ combined with a viologen-induced reactivation of the O₂ inhibited enzyme. Moreover, the viologen-modified polymer allows for electrochemical co-deposition of polymer and biocatalyst and, by this, for control of the film thickness. Protection and reactivation of the enzyme was demonstrated in thick and thin reaction layers.

Original language	English
Pages (from-to)	964-968
Number of pages	5
Journal	ACS Energy Letters
Volume	2
Issue number	5
DOIs	https://doi.org/10.1021/acsenergylett.7b00167
State	Published - 12 May 2017
Externally published	Yes

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10.1021/acsenergylett.7b00167

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title = "Protection and Reactivation of the [NiFeSe] Hydrogenase from Desulfovibrio vulgaris Hildenborough under Oxidative Conditions",

abstract = "We report on the fabrication of bioanodes for H2 oxidation based on [NiFeSe] hydrogenase. The enzyme was electrically wired by means of a specifically designed low-potential viologen-modified polymer, which delivers benchmark H2 oxidizing currents even under deactivating conditions owing to efficient protection against O2 combined with a viologen-induced reactivation of the O2 inhibited enzyme. Moreover, the viologen-modified polymer allows for electrochemical co-deposition of polymer and biocatalyst and, by this, for control of the film thickness. Protection and reactivation of the enzyme was demonstrated in thick and thin reaction layers.",

author = "Adrian Ruff and Julian Szczesny and S{\'o}nia Zacarias and Pereira, \{In{\^e}s A.C.\} and Nicolas Plumer{\'e} and Wolfgang Schuhmann",

note = "Publisher Copyright: {\textcopyright} 2017 American Chemical Society.",

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doi = "10.1021/acsenergylett.7b00167",

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T1 - Protection and Reactivation of the [NiFeSe] Hydrogenase from Desulfovibrio vulgaris Hildenborough under Oxidative Conditions

AU - Ruff, Adrian

AU - Szczesny, Julian

AU - Zacarias, Sónia

AU - Pereira, Inês A.C.

AU - Plumeré, Nicolas

AU - Schuhmann, Wolfgang

PY - 2017/5/12

Y1 - 2017/5/12

N2 - We report on the fabrication of bioanodes for H2 oxidation based on [NiFeSe] hydrogenase. The enzyme was electrically wired by means of a specifically designed low-potential viologen-modified polymer, which delivers benchmark H2 oxidizing currents even under deactivating conditions owing to efficient protection against O2 combined with a viologen-induced reactivation of the O2 inhibited enzyme. Moreover, the viologen-modified polymer allows for electrochemical co-deposition of polymer and biocatalyst and, by this, for control of the film thickness. Protection and reactivation of the enzyme was demonstrated in thick and thin reaction layers.

AB - We report on the fabrication of bioanodes for H2 oxidation based on [NiFeSe] hydrogenase. The enzyme was electrically wired by means of a specifically designed low-potential viologen-modified polymer, which delivers benchmark H2 oxidizing currents even under deactivating conditions owing to efficient protection against O2 combined with a viologen-induced reactivation of the O2 inhibited enzyme. Moreover, the viologen-modified polymer allows for electrochemical co-deposition of polymer and biocatalyst and, by this, for control of the film thickness. Protection and reactivation of the enzyme was demonstrated in thick and thin reaction layers.

UR - https://www.scopus.com/pages/publications/85033724488

U2 - 10.1021/acsenergylett.7b00167

DO - 10.1021/acsenergylett.7b00167

M3 - Article

AN - SCOPUS:85033724488

SN - 2380-8195

VL - 2

SP - 964

EP - 968

JO - ACS Energy Letters

JF - ACS Energy Letters

IS - 5

ER -

Protection and Reactivation of the [NiFeSe] Hydrogenase from Desulfovibrio vulgaris Hildenborough under Oxidative Conditions

Abstract

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