PROLIFERATION VON MEGAKARYOZYTAREN VORLAUFERZELLEN (CFU-M) IN EINEM MIKROAGARKULTURSYSTEM

Translated title of the contribution: Proliferation of megakaryocytic precursor cells in a microagar culture system

D. Geissler, Ch Peschel, J. Boyd, G. Konwalinka

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

The recently described microagar culture system for cloning erythropoietic progenitor cells was used to study the optimum conditions for the growth of CFU-M. In this system human mononuclear cells from normal human bone marrow were suspended in agar and incubated for 12 days. Various concentrations of phytohaemagglutinin lymphocyte conditioned medium (PHA-LCM) and prostaglandin E (PGE) were added to the liquid overlayer in the presence of 2-mercaptoethanol (2-ME) for the stimulation of CFU-M. Human AB serum was used instead of fetal calf serum (FCS) in all experiments. A sigmoidal dose-response curve, with a plateau at a concentration of 5 to 10%, was obtained by the addition of different concentrations of PHA-LCM in the presence of 10-6PG-E. Under optimal conditions (5% PHA-LCM, PGE 10-6M) a linear relation was obtained between the number of seeded cells and the megakaryocytic colonies formed. For routine morphological analysis the whole agar layer was stained using the Pappenheim method. For further characterization of CFU-M, an immunofluorescence test with rabbit antihuman factor VIII related antigen was performed on the whole agar layer.

Translated title of the contributionProliferation of megakaryocytic precursor cells in a microagar culture system
Original languageGerman
Pages (from-to)8-11
Number of pages4
JournalWiener Klinische Wochenschrift
Volume96
Issue number1
StatePublished - 1984
Externally publishedYes

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