Progressive loss of the spongiotrophoblast layer of Birc6/Bruce mutants results in embryonic lethality

Christiane Hitz, Daniela Vogt-Weisenhorn, Patricia Ruiz, Wolfgang Wurst, Thomas Floss

Research output: Contribution to journalArticlepeer-review

25 Scopus citations

Abstract

We have generated a mouse line with a mutant allele of the mouse Bruce/Birc6 gene induced by gene trap mutagenesis. Based on its structural features, Bruce is a member of the family of apoptosis inhibitor proteins (IAPs). This mutation leads to a truncated transcript and protein and results in a complete loss of the wildtype Bruce protein. Bruce mutant mice die from a progressive loss of their placental spongiotrophoblast layer between day 11.5 and 14.5 of embryonic development. The cause of the Bruce homozygous mutant phenotype is a lack of proliferation of spongiotrophoblast cells in the developing placenta. In contrast to in vitro data, which indicate a function for Bruce in apoptosis inhibition, the in vivo results presented here suggest instead a role for Bruce in cell division.

Original languageEnglish
Pages (from-to)91-103
Number of pages13
JournalGenesis
Volume42
Issue number2
DOIs
StatePublished - Jun 2005
Externally publishedYes

Keywords

  • Apoptosis
  • Gene trap
  • IAP
  • Spongiotrophoblast

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