Production of polyclonal and monoclonal antibodies against hyphae from arbuscular mycorrhizal fungi

Christa Göbel, A. Hahn, B. Hock

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14 Scopus citations

Abstract

Polyclonal and monoclonal antibodies were produced against hyphae of the arbuscular mycorrhizal fungus Glomus monosporum. The polyclonal antibodies (pAbs) were raised in a rabbit by immunizing with hyphae. They were tested for their specificity by a dot-immunoblot assay (DIBA). After the third immunization, a distinct difference in the signal strength was observed between the antisera and the preimmune serum. The pAbs showed cross-reactions to a number of fungal species, both mycorrhizal and other. For the production of monoclonal antibodies (mAbs), mice were immunized intraperitoneally with hyphae. The resulting hybridoma cell culture supernatants were tested by an indirect immunolabeling procedure. For this purpose the hyphae were immobilized on silane-coated microscopic slides. The mAb 8A7 reacted with hyphae from all Glomus isolates tested so far. Cross-reactivities were not observed with hyphae from fungi of the family Acaulosporaceae, phytopathogenic fungi tested so far, or from spores from Glomus species.

Original languageEnglish
Pages (from-to)293-304
Number of pages12
JournalCritical Reviews in Biotechnology
Volume15
Issue number3-4
DOIs
StatePublished - 1995

Keywords

  • Antibodies
  • Dot-immunoblot assay
  • Extraradical mycelium
  • Glomales
  • Immunofluorescence

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