TY - JOUR
T1 - Production of Lactobacillus kefir cells for asymmetric synthesis of a 3,5-dihydroxycarboxylate
AU - Pfruender, Holger
AU - Amidjojo, Maya
AU - Hang, Florian
AU - Weuster-Botz, Dirk
N1 - Funding Information:
Acknowledgements The authors are very grateful to M. Mueller for discussion and for providing us with the dioxohexanoate and hydroxyhexanoate products. We would like to thank W. Hummel and T. Daussmann (Juelich Fine Chemicals, Germany) for helpful discussions. This work was supported by the Deutsche Bundes-stiftung Umwelt (grant no. AZ 13040/03)
PY - 2005/6
Y1 - 2005/6
N2 - An efficient fedbatch process for the production of Lactobacillus kefir DSM 20587 cells was developed. An improvement in space time yield of 270% (3.7 gDCW l-1-day-1) and in final enzyme activity of 440% (9.1 U/ml) was achieved on a 150 l scale by controlling the oxygen transfer rate to 7-9 mmol l-1 h-1. The cells exhibited good and highly stereoselective reducing activities against tert-butyl 6-chloro-3,5-dioxohexanoate. tert-Butyl (3R,5S)-6-chloro-dihydroxyhexanoate, a chiral building block for HMG-CoA reductase inhibitor synthesis, was produced with 47.5% yield and >99% ee at C33 and C55 in a simple batch biotransformation process.
AB - An efficient fedbatch process for the production of Lactobacillus kefir DSM 20587 cells was developed. An improvement in space time yield of 270% (3.7 gDCW l-1-day-1) and in final enzyme activity of 440% (9.1 U/ml) was achieved on a 150 l scale by controlling the oxygen transfer rate to 7-9 mmol l-1 h-1. The cells exhibited good and highly stereoselective reducing activities against tert-butyl 6-chloro-3,5-dioxohexanoate. tert-Butyl (3R,5S)-6-chloro-dihydroxyhexanoate, a chiral building block for HMG-CoA reductase inhibitor synthesis, was produced with 47.5% yield and >99% ee at C33 and C55 in a simple batch biotransformation process.
UR - https://www.scopus.com/pages/publications/21244493671
U2 - 10.1007/s00253-004-1837-6
DO - 10.1007/s00253-004-1837-6
M3 - Article
C2 - 15614560
AN - SCOPUS:21244493671
SN - 0175-7598
VL - 67
SP - 619
EP - 622
JO - Applied Microbiology and Biotechnology
JF - Applied Microbiology and Biotechnology
IS - 5
ER -