TY - JOUR
T1 - Production of human cloned antibodies specific for hepatitis D virus-encoded small and large protein
AU - Liu, Feng
AU - Roggendorf, Michael
AU - Rasshofer, Rudolf
AU - Zachoval, Reinhart
AU - Deinhardt, Friedrich
PY - 1993
Y1 - 1993
N2 - Cloned antibodies to specific epitopes of hepatitis D virus were produced by transformation with Epstein-Barr virus and subsequent cloning of peripheral blood B lymphocytes from a patient with chronic hepatitis D virus infection. Several stable cloned B cell lines, derived from two parent cultures, produced hepatitis D-virus-specific IgG antibodies. Some cloned IgG antibodies detected hepatitis D virus-associated antigen in hepatitis D virus-infected woodchuck liver tissue sections by indirect immunofluorescence staining and some reacted in an inhibition ELISA test detecting hepatitis D virus antibodies; most cloned IgG lines detected hepatitis D antigen both in immunofluorescence tests and in inhibition ELISA. Cloned antibodies to hepatitis D antigen detected by ELISA and/or immunofluorescence staining recognized the two major specific native and denatured polypeptides, p27 and p29, in Western blot analysis. Such cloned antibodies for hepatitis D virus are potentially useful for clinical diagnosis and research.
AB - Cloned antibodies to specific epitopes of hepatitis D virus were produced by transformation with Epstein-Barr virus and subsequent cloning of peripheral blood B lymphocytes from a patient with chronic hepatitis D virus infection. Several stable cloned B cell lines, derived from two parent cultures, produced hepatitis D-virus-specific IgG antibodies. Some cloned IgG antibodies detected hepatitis D virus-associated antigen in hepatitis D virus-infected woodchuck liver tissue sections by indirect immunofluorescence staining and some reacted in an inhibition ELISA test detecting hepatitis D virus antibodies; most cloned IgG lines detected hepatitis D antigen both in immunofluorescence tests and in inhibition ELISA. Cloned antibodies to hepatitis D antigen detected by ELISA and/or immunofluorescence staining recognized the two major specific native and denatured polypeptides, p27 and p29, in Western blot analysis. Such cloned antibodies for hepatitis D virus are potentially useful for clinical diagnosis and research.
KW - Cloned antibodies
KW - Delta virus
KW - Hepatitis D polypeptides
UR - http://www.scopus.com/inward/record.url?scp=0027496006&partnerID=8YFLogxK
U2 - 10.1016/S0168-8278(05)80575-6
DO - 10.1016/S0168-8278(05)80575-6
M3 - Article
C2 - 7507955
AN - SCOPUS:0027496006
SN - 0168-8278
VL - 19
SP - 222
EP - 227
JO - Journal of Hepatology
JF - Journal of Hepatology
IS - 2
ER -
