Production and Purification of Adeno-Associated Viral Vectors for the Development of Immune-Competent Mouse Models of Persistent Hepatitis B Virus Replication

Lisa Schiller; Chunkyu Ko; Anna D. Kosinska; Dirk Grimm; Ulrike Protzer

doi:10.1007/978-1-0716-4027-2_18

Production and Purification of Adeno-Associated Viral Vectors for the Development of Immune-Competent Mouse Models of Persistent Hepatitis B Virus Replication

Lisa Schiller, Chunkyu Ko, Anna D. Kosinska, Dirk Grimm, Ulrike Protzer

Chair of Virology

Research output: Contribution to journal › Article › peer-review

Abstract

Mice infected with a recombinant adeno-associated virus carrying a replication-competent hepatitis B virus genome (rAAV-HBV) via the intravenous route establish a persistent HBV replication in hepatocytes and develop immune tolerance. They serve as models to evaluate antiviral immunity and to assess potential therapeutic approaches for chronic HBV infection. Combining selected HBV variants and different mouse genotypes allows for addressing a broad spectrum of research questions. This chapter describes the basic principles of the rAAV-HBV mouse model, rAAV-HBV production and purification methods, and finally, the in vivo application.

Original language	English
Pages (from-to)	207-218
Number of pages	12
Journal	Methods in molecular biology (Clifton, N.J.)
Volume	2837
DOIs	https://doi.org/10.1007/978-1-0716-4027-2_18
State	Published - 2024

Keywords

Adeno-associated virus
Cchronic HBV infection
Gene transfer
HBV variants
Hepatitis B virus
Iodixanol purification
Mouse model

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/978-1-0716-4027-2_18

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title = "Production and Purification of Adeno-Associated Viral Vectors for the Development of Immune-Competent Mouse Models of Persistent Hepatitis B Virus Replication",

abstract = "Mice infected with a recombinant adeno-associated virus carrying a replication-competent hepatitis B virus genome (rAAV-HBV) via the intravenous route establish a persistent HBV replication in hepatocytes and develop immune tolerance. They serve as models to evaluate antiviral immunity and to assess potential therapeutic approaches for chronic HBV infection. Combining selected HBV variants and different mouse genotypes allows for addressing a broad spectrum of research questions. This chapter describes the basic principles of the rAAV-HBV mouse model, rAAV-HBV production and purification methods, and finally, the in vivo application.",

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author = "Lisa Schiller and Chunkyu Ko and Kosinska, {Anna D.} and Dirk Grimm and Ulrike Protzer",

note = "Publisher Copyright: {\textcopyright} 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",

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doi = "10.1007/978-1-0716-4027-2_18",

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AU - Ko, Chunkyu

AU - Kosinska, Anna D.

AU - Grimm, Dirk

AU - Protzer, Ulrike

PY - 2024

Y1 - 2024

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AB - Mice infected with a recombinant adeno-associated virus carrying a replication-competent hepatitis B virus genome (rAAV-HBV) via the intravenous route establish a persistent HBV replication in hepatocytes and develop immune tolerance. They serve as models to evaluate antiviral immunity and to assess potential therapeutic approaches for chronic HBV infection. Combining selected HBV variants and different mouse genotypes allows for addressing a broad spectrum of research questions. This chapter describes the basic principles of the rAAV-HBV mouse model, rAAV-HBV production and purification methods, and finally, the in vivo application.

KW - Adeno-associated virus

KW - Cchronic HBV infection

KW - Gene transfer

KW - HBV variants

KW - Hepatitis B virus

KW - Iodixanol purification

KW - Mouse model

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SN - 1064-3745

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JO - Methods in molecular biology (Clifton, N.J.)

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Production and Purification of Adeno-Associated Viral Vectors for the Development of Immune-Competent Mouse Models of Persistent Hepatitis B Virus Replication

Abstract

Keywords

UN SDGs

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