Probing whole cell currents in high-frequency electrical fields: Identification of thermal effects

Michael Olapinski; Stephan Manus; Niels Fertig; Friedrich C. Simmel

doi:10.1016/j.bios.2007.09.004

Probing whole cell currents in high-frequency electrical fields: Identification of thermal effects

Michael Olapinski, Stephan Manus, Niels Fertig, Friedrich C. Simmel

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

An open-end coaxial probe is combined with a planar patch-clamp system to apply electric fields with GHz frequencies during conventional patch-clamp measurements. The combination of pulsed microwave irradiation and lock-in detection allows for the separation of fast and slow effects and hence facilitates the identification of thermal effects. The setup and the influence of radiation on the patch-clamp current are thoroughly characterized. For the independent optical verification of heating effects, a temperature microscopy technique is applied with high spatial, temporal and temperature resolution. It is shown that the effect of radiation at GHz frequencies on whole cell currents is predominantly thermal in nature in the case of RBL cells with an endogenous K_ir 2.1 channel.

Original language	English
Pages (from-to)	872-878
Number of pages	7
Journal	Biosensors and Bioelectronics
Volume	23
Issue number	6
DOIs	https://doi.org/10.1016/j.bios.2007.09.004
State	Published - 18 Jan 2008
Externally published	Yes

Keywords

Biosensor
Electrophysiology
High-frequency techniques
Lab on a chip
Patch-clamp

Access to Document

10.1016/j.bios.2007.09.004

Cite this

@article{597a3c29b9b4427793424fd7cb71dbf9,

title = "Probing whole cell currents in high-frequency electrical fields: Identification of thermal effects",

abstract = "An open-end coaxial probe is combined with a planar patch-clamp system to apply electric fields with GHz frequencies during conventional patch-clamp measurements. The combination of pulsed microwave irradiation and lock-in detection allows for the separation of fast and slow effects and hence facilitates the identification of thermal effects. The setup and the influence of radiation on the patch-clamp current are thoroughly characterized. For the independent optical verification of heating effects, a temperature microscopy technique is applied with high spatial, temporal and temperature resolution. It is shown that the effect of radiation at GHz frequencies on whole cell currents is predominantly thermal in nature in the case of RBL cells with an endogenous Kir 2.1 channel.",

keywords = "Biosensor, Electrophysiology, High-frequency techniques, Lab on a chip, Patch-clamp",

author = "Michael Olapinski and Stephan Manus and Niels Fertig and Simmel, {Friedrich C.}",

note = "Funding Information: The authors thank J.P. Kotthaus, J. Behrends, G. Schwake, S. Kempter and J.O. R{\"a}dler for help and useful discussions. Financial support by the BMBF (BMBF 13N8363, 13N8364) and the DFG (SI 761/2-1/2/3) is gratefully acknowledged. M.O. is supported by the IDK-NBT Munich. ",

year = "2008",

month = jan,

day = "18",

doi = "10.1016/j.bios.2007.09.004",

language = "English",

volume = "23",

pages = "872--878",

journal = "Biosensors and Bioelectronics",

issn = "0956-5663",

publisher = "Elsevier Ltd.",

number = "6",

}

TY - JOUR

T1 - Probing whole cell currents in high-frequency electrical fields

T2 - Identification of thermal effects

AU - Olapinski, Michael

AU - Manus, Stephan

AU - Fertig, Niels

AU - Simmel, Friedrich C.

N1 - Funding Information: The authors thank J.P. Kotthaus, J. Behrends, G. Schwake, S. Kempter and J.O. Rädler for help and useful discussions. Financial support by the BMBF (BMBF 13N8363, 13N8364) and the DFG (SI 761/2-1/2/3) is gratefully acknowledged. M.O. is supported by the IDK-NBT Munich.

PY - 2008/1/18

Y1 - 2008/1/18

N2 - An open-end coaxial probe is combined with a planar patch-clamp system to apply electric fields with GHz frequencies during conventional patch-clamp measurements. The combination of pulsed microwave irradiation and lock-in detection allows for the separation of fast and slow effects and hence facilitates the identification of thermal effects. The setup and the influence of radiation on the patch-clamp current are thoroughly characterized. For the independent optical verification of heating effects, a temperature microscopy technique is applied with high spatial, temporal and temperature resolution. It is shown that the effect of radiation at GHz frequencies on whole cell currents is predominantly thermal in nature in the case of RBL cells with an endogenous Kir 2.1 channel.

AB - An open-end coaxial probe is combined with a planar patch-clamp system to apply electric fields with GHz frequencies during conventional patch-clamp measurements. The combination of pulsed microwave irradiation and lock-in detection allows for the separation of fast and slow effects and hence facilitates the identification of thermal effects. The setup and the influence of radiation on the patch-clamp current are thoroughly characterized. For the independent optical verification of heating effects, a temperature microscopy technique is applied with high spatial, temporal and temperature resolution. It is shown that the effect of radiation at GHz frequencies on whole cell currents is predominantly thermal in nature in the case of RBL cells with an endogenous Kir 2.1 channel.

KW - Biosensor

KW - Electrophysiology

KW - High-frequency techniques

KW - Lab on a chip

KW - Patch-clamp

UR - http://www.scopus.com/inward/record.url?scp=37349080564&partnerID=8YFLogxK

U2 - 10.1016/j.bios.2007.09.004

DO - 10.1016/j.bios.2007.09.004

M3 - Article

C2 - 17959369

AN - SCOPUS:37349080564

SN - 0956-5663

VL - 23

SP - 872

EP - 878

JO - Biosensors and Bioelectronics

JF - Biosensors and Bioelectronics

IS - 6

ER -

Probing whole cell currents in high-frequency electrical fields: Identification of thermal effects

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this