Probing lipid membranes and ion channels with high frequency spectroscopy

Michael Olapinski, Andrea Bruggemann, Michael George, Stephan Manus, Niels Fertig, Friedrich C. Simmel

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

A coplanar waveguide defined on a glass chip is integrated within a microfluidic cartridge to allow for high-frequency testing of lipid bilayer membranes. The membranes are carefully deposited on the circuit using a solvent exchange method or, alternatively, vesicle fusion. The quality of the lipid bilayer membranes is controlled by fluorescence microscopy and scanning probe techniques. The deposition of the bilayers results in a significant change in the high frequency transmission properties of the circuit. We also embed alamethicin, a model ion channel forming peptide, in the bilayers in order to quantify the influence of channel charging events on the overall impedance.

Original languageEnglish
Title of host publication2004 4th IEEE Conference on Nanotechnology
Pages565-567
Number of pages3
StatePublished - 2004
Externally publishedYes
Event2004 4th IEEE Conference on Nanotechnology - Munich, Germany
Duration: 16 Aug 200419 Aug 2004

Publication series

Name2004 4th IEEE Conference on Nanotechnology

Conference

Conference2004 4th IEEE Conference on Nanotechnology
Country/TerritoryGermany
CityMunich
Period16/08/0419/08/04

Keywords

  • High frequency
  • Impedance spectroscopy
  • Ion channels
  • Lipid bilayers
  • Nanobiotechnology

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