TY - JOUR
T1 - Primary and Secondary Metabolites in Lotus japonicus
AU - Ranner, Josef L.
AU - Schalk, Sabrina
AU - Martyniak, Cindy
AU - Parniske, Martin
AU - Gutjahr, Caroline
AU - Stark, Timo D.
AU - Dawid, Corinna
N1 - Publisher Copyright:
© 2023 The Authors. Published by American Chemical Society.
PY - 2023/8/2
Y1 - 2023/8/2
N2 - Lotus japonicus is a leguminous model plant used to gain insight into plant physiology, stress response, and especially symbiotic plant-microbe interactions, such as root nodule symbiosis or arbuscular mycorrhiza. Responses to changing environmental conditions, stress, microbes, or insect pests are generally accompanied by changes in primary and secondary metabolism to account for physiological needs or to produce defensive or signaling compounds. Here we provide an overview of the primary and secondary metabolites identified in L. japonicus to date. Identification of the metabolites is mainly based on mass spectral tags (MSTs) obtained by gas chromatography linked with tandem mass spectrometry (GC-MS/MS) or liquid chromatography-MS/MS (LC-MS/MS). These MSTs contain retention index and mass spectral information, which are compared to databases with MSTs of authentic standards. More than 600 metabolites are grouped into compound classes such as polyphenols, carbohydrates, organic acids and phosphates, lipids, amino acids, nitrogenous compounds, phytohormones, and additional defense compounds. Their physiological effects are briefly discussed, and the detection methods are explained. This review of the exisiting literature on L. japonicus metabolites provides a valuable basis for future metabolomics studies.
AB - Lotus japonicus is a leguminous model plant used to gain insight into plant physiology, stress response, and especially symbiotic plant-microbe interactions, such as root nodule symbiosis or arbuscular mycorrhiza. Responses to changing environmental conditions, stress, microbes, or insect pests are generally accompanied by changes in primary and secondary metabolism to account for physiological needs or to produce defensive or signaling compounds. Here we provide an overview of the primary and secondary metabolites identified in L. japonicus to date. Identification of the metabolites is mainly based on mass spectral tags (MSTs) obtained by gas chromatography linked with tandem mass spectrometry (GC-MS/MS) or liquid chromatography-MS/MS (LC-MS/MS). These MSTs contain retention index and mass spectral information, which are compared to databases with MSTs of authentic standards. More than 600 metabolites are grouped into compound classes such as polyphenols, carbohydrates, organic acids and phosphates, lipids, amino acids, nitrogenous compounds, phytohormones, and additional defense compounds. Their physiological effects are briefly discussed, and the detection methods are explained. This review of the exisiting literature on L. japonicus metabolites provides a valuable basis for future metabolomics studies.
KW - Lotus japonicus
KW - mass spectral tags
KW - metabolomics
KW - phytohormones
KW - plant−microbe interactions
KW - primary metabolites
KW - secondary metabolites
UR - http://www.scopus.com/inward/record.url?scp=85166387267&partnerID=8YFLogxK
U2 - 10.1021/acs.jafc.3c02709
DO - 10.1021/acs.jafc.3c02709
M3 - Review article
C2 - 37466334
AN - SCOPUS:85166387267
SN - 0021-8561
VL - 71
SP - 11277
EP - 11303
JO - Journal of agricultural and food chemistry
JF - Journal of agricultural and food chemistry
IS - 30
ER -