Prevalence of hepatitis E virus antibodies in children in Germany

Andi Krumbholz; Anne Neubert; Sebastian Joel; Hermann Girschick; Hans Iko Huppertz; Petra Kaiser; Johannes Liese; Andrea Streng; Tim Niehues; Jochen Peters; Axel Sauerbrey; Horst Schroten; Tobias Tenenbaum; Stefan Wirth; Roland Zell; Andreas Sauerbrei

doi:10.1097/INF.0000000000000077

Prevalence of hepatitis E virus antibodies in children in Germany

Andi Krumbholz
, Anne Neubert
, Sebastian Joel
, Hermann Girschick
, Hans Iko Huppertz
, Petra Kaiser
, Johannes Liese
, Andrea Streng
, Tim Niehues
, Jochen Peters
, Axel Sauerbrey
, Horst Schroten
, Tobias Tenenbaum
, Stefan Wirth
, Roland Zell
, Andreas Sauerbrei

Friedrich Schiller University Jena

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

RESULTS: In children, the overall prevalence of antibodies was 1.0%. Starting with the 5- to 6-year olds, there was a significant increase of HEV seroprevalence to 1.5% in the group of the 15- to 17-year olds. There was no statistically significant difference between seroprevalences of boys (1.2%) and girls (0.7%). Passively transmitted maternal antibodies persisted for about 3 months. The strength of agreement between the recomWell/recomLine system and the ELISAs from MP Biomedicals or Axiom varied between 0.229 and 0.542 and was calculated at 0.111 when the assays from MP Biomedicals and Axiom were compared.

CONCLUSIONS: In Germany, only a very small number of HEV infections occur in children. Many infections occur in adults with increasing age. Because of considerable variations in assay accordance, there is an urgent need for standardization of HEV serology.

BACKGROUND: Since asymptomatic hepatitis E virus (HEV) infections particularly affect children, there is a need for studies to determine the HEV seroprevalence among infants, children and adolescents.

METHODS: The prevalence of anti-HEV IgG antibodies was determined in sera taken in 2008-2010 from 1646 children aged 0-17 years living in Germany. Antibody testing was carried out using the enzyme-linked immunosorbent assay recomWell HEV IgG as well as the recomLine HEV IgG/IgM distributed by Mikrogen. Furthermore, the performance of MP Biomedicals enzyme-linked immunosorbent assay HEV and the HEV-Ab enzyme-linked immunosorbent assay from Axiom was analyzed in comparison with the recomWell/recomLine test system using a defined subset of sera.

Original language	English
Pages (from-to)	258-262
Number of pages	5
Journal	Pediatric Infectious Disease Journal
Volume	33
Issue number	3
DOIs	https://doi.org/10.1097/INF.0000000000000077
State	Published - 1 Mar 2014
Externally published	Yes

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10.1097/INF.0000000000000077

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Krumbholz, A., Neubert, A., Joel, S., Girschick, H., Huppertz, H. I., Kaiser, P., Liese, J., Streng, A., Niehues, T., Peters, J., Sauerbrey, A., Schroten, H., Tenenbaum, T., Wirth, S., Zell, R., & Sauerbrei, A. (2014). Prevalence of hepatitis E virus antibodies in children in Germany. Pediatric Infectious Disease Journal, 33(3), 258-262. https://doi.org/10.1097/INF.0000000000000077

@article{ec0f6e1521324c8cacffbc4df907529f,

title = "Prevalence of hepatitis E virus antibodies in children in Germany",

abstract = "RESULTS: In children, the overall prevalence of antibodies was 1.0\%. Starting with the 5- to 6-year olds, there was a significant increase of HEV seroprevalence to 1.5\% in the group of the 15- to 17-year olds. There was no statistically significant difference between seroprevalences of boys (1.2\%) and girls (0.7\%). Passively transmitted maternal antibodies persisted for about 3 months. The strength of agreement between the recomWell/recomLine system and the ELISAs from MP Biomedicals or Axiom varied between 0.229 and 0.542 and was calculated at 0.111 when the assays from MP Biomedicals and Axiom were compared.CONCLUSIONS: In Germany, only a very small number of HEV infections occur in children. Many infections occur in adults with increasing age. Because of considerable variations in assay accordance, there is an urgent need for standardization of HEV serology.BACKGROUND: Since asymptomatic hepatitis E virus (HEV) infections particularly affect children, there is a need for studies to determine the HEV seroprevalence among infants, children and adolescents.METHODS: The prevalence of anti-HEV IgG antibodies was determined in sera taken in 2008-2010 from 1646 children aged 0-17 years living in Germany. Antibody testing was carried out using the enzyme-linked immunosorbent assay recomWell HEV IgG as well as the recomLine HEV IgG/IgM distributed by Mikrogen. Furthermore, the performance of MP Biomedicals enzyme-linked immunosorbent assay HEV and the HEV-Ab enzyme-linked immunosorbent assay from Axiom was analyzed in comparison with the recomWell/recomLine test system using a defined subset of sera.",

author = "Andi Krumbholz and Anne Neubert and Sebastian Joel and Hermann Girschick and Huppertz, \{Hans Iko\} and Petra Kaiser and Johannes Liese and Andrea Streng and Tim Niehues and Jochen Peters and Axel Sauerbrey and Horst Schroten and Tobias Tenenbaum and Stefan Wirth and Roland Zell and Andreas Sauerbrei",

year = "2014",

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doi = "10.1097/INF.0000000000000077",

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Krumbholz, A, Neubert, A, Joel, S, Girschick, H, Huppertz, HI, Kaiser, P, Liese, J, Streng, A, Niehues, T, Peters, J, Sauerbrey, A, Schroten, H, Tenenbaum, T, Wirth, S, Zell, R & Sauerbrei, A 2014, 'Prevalence of hepatitis E virus antibodies in children in Germany', Pediatric Infectious Disease Journal, vol. 33, no. 3, pp. 258-262. https://doi.org/10.1097/INF.0000000000000077

TY - JOUR

T1 - Prevalence of hepatitis E virus antibodies in children in Germany

AU - Krumbholz, Andi

AU - Neubert, Anne

AU - Joel, Sebastian

AU - Girschick, Hermann

AU - Huppertz, Hans Iko

AU - Kaiser, Petra

AU - Liese, Johannes

AU - Streng, Andrea

AU - Niehues, Tim

AU - Peters, Jochen

AU - Sauerbrey, Axel

AU - Schroten, Horst

AU - Tenenbaum, Tobias

AU - Wirth, Stefan

AU - Zell, Roland

AU - Sauerbrei, Andreas

PY - 2014/3/1

Y1 - 2014/3/1

N2 - RESULTS: In children, the overall prevalence of antibodies was 1.0%. Starting with the 5- to 6-year olds, there was a significant increase of HEV seroprevalence to 1.5% in the group of the 15- to 17-year olds. There was no statistically significant difference between seroprevalences of boys (1.2%) and girls (0.7%). Passively transmitted maternal antibodies persisted for about 3 months. The strength of agreement between the recomWell/recomLine system and the ELISAs from MP Biomedicals or Axiom varied between 0.229 and 0.542 and was calculated at 0.111 when the assays from MP Biomedicals and Axiom were compared.CONCLUSIONS: In Germany, only a very small number of HEV infections occur in children. Many infections occur in adults with increasing age. Because of considerable variations in assay accordance, there is an urgent need for standardization of HEV serology.BACKGROUND: Since asymptomatic hepatitis E virus (HEV) infections particularly affect children, there is a need for studies to determine the HEV seroprevalence among infants, children and adolescents.METHODS: The prevalence of anti-HEV IgG antibodies was determined in sera taken in 2008-2010 from 1646 children aged 0-17 years living in Germany. Antibody testing was carried out using the enzyme-linked immunosorbent assay recomWell HEV IgG as well as the recomLine HEV IgG/IgM distributed by Mikrogen. Furthermore, the performance of MP Biomedicals enzyme-linked immunosorbent assay HEV and the HEV-Ab enzyme-linked immunosorbent assay from Axiom was analyzed in comparison with the recomWell/recomLine test system using a defined subset of sera.

AB - RESULTS: In children, the overall prevalence of antibodies was 1.0%. Starting with the 5- to 6-year olds, there was a significant increase of HEV seroprevalence to 1.5% in the group of the 15- to 17-year olds. There was no statistically significant difference between seroprevalences of boys (1.2%) and girls (0.7%). Passively transmitted maternal antibodies persisted for about 3 months. The strength of agreement between the recomWell/recomLine system and the ELISAs from MP Biomedicals or Axiom varied between 0.229 and 0.542 and was calculated at 0.111 when the assays from MP Biomedicals and Axiom were compared.CONCLUSIONS: In Germany, only a very small number of HEV infections occur in children. Many infections occur in adults with increasing age. Because of considerable variations in assay accordance, there is an urgent need for standardization of HEV serology.BACKGROUND: Since asymptomatic hepatitis E virus (HEV) infections particularly affect children, there is a need for studies to determine the HEV seroprevalence among infants, children and adolescents.METHODS: The prevalence of anti-HEV IgG antibodies was determined in sera taken in 2008-2010 from 1646 children aged 0-17 years living in Germany. Antibody testing was carried out using the enzyme-linked immunosorbent assay recomWell HEV IgG as well as the recomLine HEV IgG/IgM distributed by Mikrogen. Furthermore, the performance of MP Biomedicals enzyme-linked immunosorbent assay HEV and the HEV-Ab enzyme-linked immunosorbent assay from Axiom was analyzed in comparison with the recomWell/recomLine test system using a defined subset of sera.

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U2 - 10.1097/INF.0000000000000077

DO - 10.1097/INF.0000000000000077

M3 - Article

C2 - 24168981

AN - SCOPUS:85027933751

SN - 0891-3668

VL - 33

SP - 258

EP - 262

JO - Pediatric Infectious Disease Journal

JF - Pediatric Infectious Disease Journal

IS - 3

ER -

Prevalence of hepatitis E virus antibodies in children in Germany

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