Abstract
Immunoreactive angiotensin I and angiotensin II were found in human urine that was purified on octadecasilylsilica cartridges. The daily excretion of angiotensin I and II in healthy volunteers was 189.00 (SE 38.36) and 17.54 (SE 3.07) pmol/24 h or 148.09 (SE 32.22) and 12.82 (SE 2.34) pmol/L, respectively (n = 12). No circadian rhythm was observed in the excretion patterns of angiotensin I and II. In vitro degradation of angiotensin I or II could not be detected in acidified urine samples. A marked increase in the excretion of angiotensin I and II could be demonstrated in patients with anaphylactoid reactions to drugs and food additives after oral challenge. Immunoreactive angiotensin I and II could be characterized by HPLC as IIe5-angiotensin I, IIe5-angiotensin II, and angiotensin II metabolites.
Original language | English |
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Pages (from-to) | 1768-1772 |
Number of pages | 5 |
Journal | Clinical Chemistry |
Volume | 38 |
Issue number | 9 |
State | Published - 1992 |
Externally published | Yes |
Keywords
- Anaphylaxis
- Chromatography
- Radioimmunoassay
- Reversed-phase
- Sample handling