Preparation of 2'-deoxy-2'-methylseleno-modified phosphoramidites and RNA.

Ronald Micura; Claudia Höbartner; Renate Rieder; Christoph Kreutz; Barbara Puffer; Kathrin Lang; Holger Moroder

Preparation of 2'-deoxy-2'-methylseleno-modified phosphoramidites and RNA.

Ronald Micura, Claudia Höbartner, Renate Rieder, Christoph Kreutz, Barbara Puffer, Kathrin Lang, Holger Moroder

University of Innsbruck

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

The derivatization of nucleic acids with selenium is a useful approach to facilitate phase determination during three-dimensional structural analysis by X-ray crystallography. This unit describes (1) the synthesis and characterization of 2'-deoxy-2'-methylseleno (2'-Se-methyl) nucleosides and their corresponding 3'-O-(2-cyanoethyl)-N,N-diisopropylphosphoramidite derivatives, (2) the site-specific incorporation of 2'-Se-methyl ribonucleosides into oligoribonucleotides by chemical RNA solid-phase synthesis, and (3) the enzymatic ligation of Se-containing RNA oligonucleotides to produce a biologically relevant RNA sequence.

Original language	English
Pages (from-to)	Unit 1.15
Journal	Current Protocols in Nucleic Acid Chemistry
Volume	Chapter 1
State	Published - Jan 2007
Externally published	Yes

Cite this

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title = "Preparation of 2'-deoxy-2'-methylseleno-modified phosphoramidites and RNA.",

abstract = "The derivatization of nucleic acids with selenium is a useful approach to facilitate phase determination during three-dimensional structural analysis by X-ray crystallography. This unit describes (1) the synthesis and characterization of 2'-deoxy-2'-methylseleno (2'-Se-methyl) nucleosides and their corresponding 3'-O-(2-cyanoethyl)-N,N-diisopropylphosphoramidite derivatives, (2) the site-specific incorporation of 2'-Se-methyl ribonucleosides into oligoribonucleotides by chemical RNA solid-phase synthesis, and (3) the enzymatic ligation of Se-containing RNA oligonucleotides to produce a biologically relevant RNA sequence.",

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T1 - Preparation of 2'-deoxy-2'-methylseleno-modified phosphoramidites and RNA.

AU - Micura, Ronald

AU - Höbartner, Claudia

AU - Rieder, Renate

AU - Kreutz, Christoph

AU - Puffer, Barbara

AU - Lang, Kathrin

AU - Moroder, Holger

PY - 2007/1

Y1 - 2007/1

N2 - The derivatization of nucleic acids with selenium is a useful approach to facilitate phase determination during three-dimensional structural analysis by X-ray crystallography. This unit describes (1) the synthesis and characterization of 2'-deoxy-2'-methylseleno (2'-Se-methyl) nucleosides and their corresponding 3'-O-(2-cyanoethyl)-N,N-diisopropylphosphoramidite derivatives, (2) the site-specific incorporation of 2'-Se-methyl ribonucleosides into oligoribonucleotides by chemical RNA solid-phase synthesis, and (3) the enzymatic ligation of Se-containing RNA oligonucleotides to produce a biologically relevant RNA sequence.

AB - The derivatization of nucleic acids with selenium is a useful approach to facilitate phase determination during three-dimensional structural analysis by X-ray crystallography. This unit describes (1) the synthesis and characterization of 2'-deoxy-2'-methylseleno (2'-Se-methyl) nucleosides and their corresponding 3'-O-(2-cyanoethyl)-N,N-diisopropylphosphoramidite derivatives, (2) the site-specific incorporation of 2'-Se-methyl ribonucleosides into oligoribonucleotides by chemical RNA solid-phase synthesis, and (3) the enzymatic ligation of Se-containing RNA oligonucleotides to produce a biologically relevant RNA sequence.

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M3 - Article

C2 - 18428963

AN - SCOPUS:48849107338

SN - 1934-9270

VL - Chapter 1

SP - Unit 1.15

JO - Current Protocols in Nucleic Acid Chemistry

JF - Current Protocols in Nucleic Acid Chemistry

ER -

Preparation of 2'-deoxy-2'-methylseleno-modified phosphoramidites and RNA.

Abstract

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