Preparation of 2'-deoxy-2'-methylseleno-modified phosphoramidites and RNA.

Ronald Micura, Claudia Höbartner, Renate Rieder, Christoph Kreutz, Barbara Puffer, Kathrin Lang, Holger Moroder

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3 Scopus citations


The derivatization of nucleic acids with selenium is a useful approach to facilitate phase determination during three-dimensional structural analysis by X-ray crystallography. This unit describes (1) the synthesis and characterization of 2'-deoxy-2'-methylseleno (2'-Se-methyl) nucleosides and their corresponding 3'-O-(2-cyanoethyl)-N,N-diisopropylphosphoramidite derivatives, (2) the site-specific incorporation of 2'-Se-methyl ribonucleosides into oligoribonucleotides by chemical RNA solid-phase synthesis, and (3) the enzymatic ligation of Se-containing RNA oligonucleotides to produce a biologically relevant RNA sequence.

Original languageEnglish
Pages (from-to)Unit 1.15
JournalCurrent Protocols in Nucleic Acid Chemistry
VolumeChapter 1
StatePublished - Jan 2007
Externally publishedYes


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