Prediction of mitochondrial protein function by comparative physiology and phylogenetic profiling

Yiming Cheng, Fabiana Perocchi

Research output: Contribution to journalArticlepeer-review

1 Scopus citations


According to the endosymbiotic theory, mitochondria originate from a free-living alpha-proteobacteria that established an intracellular symbiosis with the ancestor of present-day eukaryotic cells. During the bacterium-to-organelle transformation, the proto-mitochondrial proteome has undergone a massive turnover, whereby less than 20 % of modern mitochondrial proteomes can be traced back to the bacterial ancestor. Moreover, mitochondrial proteomes from several eukaryotic organisms, for example, yeast and human, show a rather modest overlap, reflecting differences in mitochondrial physiology. Those differences may result from the combination of differential gain and loss of genes and retargeting processes among lineages. Therefore, an evolutionary signature, also called "phylogenetic profile", could be generated for every mitochondrial protein. Here, we present two evolutionary biology approaches to study mitochondrial physiology: the first strategy, which we refer to as "comparative physiology," allows the de novo identification of mitochondrial proteins involved in a physiological function; the second, known as "phylogenetic profiling," allows to predict protein functions and functional interactions by comparing phylogenetic profiles of uncharacterized and known components.

Original languageEnglish
Pages (from-to)321-329
Number of pages9
JournalMethods in molecular biology (Clifton, N.J.)
StatePublished - 2015
Externally publishedYes


Dive into the research topics of 'Prediction of mitochondrial protein function by comparative physiology and phylogenetic profiling'. Together they form a unique fingerprint.

Cite this