Precise Correction of Heterozygous SHOX2 Mutations in hiPSCs Derived from Patients with Atrial Fibrillation via Genome Editing and Sib Selection

Simon Alexander Sumer, Sandra Hoffmann, Svenja Laue, Birgit Campbell, Kristin Raedecke, Viktoria Frajs, Sebastian Clauss, Stefan Kääb, Johannes W.G. Janssen, Anna Jauch, Karl Ludwig Laugwitz, Tatjana Dorn, Alessandra Moretti, Gudrun A. Rappold

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

In this study, Sumer, Hoffmann, et al., developed a strategy for the isolation of extremely rare hiPSC clones, suitable for scarless correction of heterozygous mutations by random enrichment of precisely edited cells and their detection via allele quantification. This strategy facilitates hiPSC-based gene correction regardless of the gene-editing approach.

Original languageEnglish
Pages (from-to)999-1013
Number of pages15
JournalStem Cell Reports
Volume15
Issue number4
DOIs
StatePublished - 13 Oct 2020
Externally publishedYes

Keywords

  • SHOX2
  • atrial fibrillation
  • isogenic control
  • patient-derived iPSCs
  • precise gene editing
  • sib selection

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