Posttranscriptional spin labeling of RNA by tetrazine-based cycloaddition

Christof Domnick; Gregor Hagelueken; Frank Eggert; Olav Schiemann; Stephanie Kath-Schorr

doi:10.1039/c8ob02597e

Posttranscriptional spin labeling of RNA by tetrazine-based cycloaddition

Christof Domnick, Gregor Hagelueken, Frank Eggert, Olav Schiemann, Stephanie Kath-Schorr

University of Bonn

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

The site-specific introduction of spin labels into RNA for distance measurements by EPR gives insight into its solution structure. We here present a method for spin labeling of in vitro transcribed RNA. Distance distributions between two nitroxide spin labels are determined by PELDOR in a self-complementary RNA duplex.

Original language	English
Pages (from-to)	1805-1808
Number of pages	4
Journal	Organic and Biomolecular Chemistry
Volume	17
Issue number	7
DOIs	https://doi.org/10.1039/c8ob02597e
State	Published - 2019
Externally published	Yes

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title = "Posttranscriptional spin labeling of RNA by tetrazine-based cycloaddition",

abstract = "The site-specific introduction of spin labels into RNA for distance measurements by EPR gives insight into its solution structure. We here present a method for spin labeling of in vitro transcribed RNA. Distance distributions between two nitroxide spin labels are determined by PELDOR in a self-complementary RNA duplex.",

author = "Christof Domnick and Gregor Hagelueken and Frank Eggert and Olav Schiemann and Stephanie Kath-Schorr",

note = "Publisher Copyright: {\textcopyright} 2019 The Royal Society of Chemistry.",

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doi = "10.1039/c8ob02597e",

language = "English",

volume = "17",

pages = "1805--1808",

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T1 - Posttranscriptional spin labeling of RNA by tetrazine-based cycloaddition

AU - Domnick, Christof

AU - Hagelueken, Gregor

AU - Eggert, Frank

AU - Schiemann, Olav

AU - Kath-Schorr, Stephanie

PY - 2019

Y1 - 2019

N2 - The site-specific introduction of spin labels into RNA for distance measurements by EPR gives insight into its solution structure. We here present a method for spin labeling of in vitro transcribed RNA. Distance distributions between two nitroxide spin labels are determined by PELDOR in a self-complementary RNA duplex.

AB - The site-specific introduction of spin labels into RNA for distance measurements by EPR gives insight into its solution structure. We here present a method for spin labeling of in vitro transcribed RNA. Distance distributions between two nitroxide spin labels are determined by PELDOR in a self-complementary RNA duplex.

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U2 - 10.1039/c8ob02597e

DO - 10.1039/c8ob02597e

M3 - Article

C2 - 30520916

AN - SCOPUS:85061511498

SN - 1477-0520

VL - 17

SP - 1805

EP - 1808

JO - Organic and Biomolecular Chemistry

JF - Organic and Biomolecular Chemistry

IS - 7

ER -

Posttranscriptional spin labeling of RNA by tetrazine-based cycloaddition

Abstract

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