Porcine familial adenomatous polyposis model enables systematic analysis of early events in adenoma progression

Tatiana Flisikowska; Monika Stachowiak; Hongen Xu; Alexandra Wagner; Alejandra Hernandez-Caceres; Christine Wurmser; Carolin Perleberg; Hubert Pausch; Anna Perkowska; Konrad Fischer; Dmitrij Frishman; Ruedi Fries; Marek Switonski; Alexander Kind; Dieter Saur; Angelika Schnieke; Krzysztof Flisikowski

doi:10.1038/s41598-017-06741-8

Porcine familial adenomatous polyposis model enables systematic analysis of early events in adenoma progression

Tatiana Flisikowska
, Monika Stachowiak
, Hongen Xu
, Alexandra Wagner
, Alejandra Hernandez-Caceres
, Christine Wurmser
, Carolin Perleberg
, Hubert Pausch
, Anna Perkowska
, Konrad Fischer
, Dmitrij Frishman
, Ruedi Fries
, Marek Switonski
, Alexander Kind
, Dieter Saur
, Angelika Schnieke
, Krzysztof Flisikowski

Technical University of Munich
Poznan University of Life Sciences
Helmholtz Zentrum München German Research Center for Environmental Health
St. Petersburg Technical University

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

We compared gene expression in low and high-grade intraepithelial dysplastic polyps from pigs carrying an APC ¹³¹¹ truncating mutation orthologous to human APC ¹³⁰⁹, analysing whole samples and microdissected dysplastic epithelium. Gene set enrichment analysis revealed differential expression of gene sets similar to human normal mucosa versus T1 stage polyps. Transcriptome analysis of whole samples revealed many differentially-expressed genes reflecting immune infiltration. Analysis of microdissected dysplastic epithelium was markedly different and showed increased expression in high-grade intraepithelial neoplasia of several genes known to be involved in human CRC; and revealed possible new roles for GBP6 and PLXND1. The pig model thus facilitates analysis of CRC pathogenesis.

Original language	English
Article number	6613
Journal	Scientific Reports
Volume	7
Issue number	1
DOIs	https://doi.org/10.1038/s41598-017-06741-8
State	Published - 1 Dec 2017

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Flisikowska, T., Stachowiak, M., Xu, H., Wagner, A., Hernandez-Caceres, A., Wurmser, C., Perleberg, C., Pausch, H., Perkowska, A., Fischer, K., Frishman, D., Fries, R., Switonski, M., Kind, A., Saur, D., Schnieke, A., & Flisikowski, K. (2017). Porcine familial adenomatous polyposis model enables systematic analysis of early events in adenoma progression. Scientific Reports, 7(1), Article 6613. https://doi.org/10.1038/s41598-017-06741-8

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title = "Porcine familial adenomatous polyposis model enables systematic analysis of early events in adenoma progression",

abstract = "We compared gene expression in low and high-grade intraepithelial dysplastic polyps from pigs carrying an APC 1311 truncating mutation orthologous to human APC 1309, analysing whole samples and microdissected dysplastic epithelium. Gene set enrichment analysis revealed differential expression of gene sets similar to human normal mucosa versus T1 stage polyps. Transcriptome analysis of whole samples revealed many differentially-expressed genes reflecting immune infiltration. Analysis of microdissected dysplastic epithelium was markedly different and showed increased expression in high-grade intraepithelial neoplasia of several genes known to be involved in human CRC; and revealed possible new roles for GBP6 and PLXND1. The pig model thus facilitates analysis of CRC pathogenesis.",

author = "Tatiana Flisikowska and Monika Stachowiak and Hongen Xu and Alexandra Wagner and Alejandra Hernandez-Caceres and Christine Wurmser and Carolin Perleberg and Hubert Pausch and Anna Perkowska and Konrad Fischer and Dmitrij Frishman and Ruedi Fries and Marek Switonski and Alexander Kind and Dieter Saur and Angelika Schnieke and Krzysztof Flisikowski",

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doi = "10.1038/s41598-017-06741-8",

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Flisikowska, T, Stachowiak, M, Xu, H, Wagner, A, Hernandez-Caceres, A, Wurmser, C, Perleberg, C, Pausch, H, Perkowska, A, Fischer, K, Frishman, D, Fries, R, Switonski, M, Kind, A, Saur, D , Schnieke, A & Flisikowski, K 2017, 'Porcine familial adenomatous polyposis model enables systematic analysis of early events in adenoma progression', Scientific Reports, vol. 7, no. 1, 6613. https://doi.org/10.1038/s41598-017-06741-8

TY - JOUR

T1 - Porcine familial adenomatous polyposis model enables systematic analysis of early events in adenoma progression

AU - Flisikowska, Tatiana

AU - Stachowiak, Monika

AU - Xu, Hongen

AU - Wagner, Alexandra

AU - Hernandez-Caceres, Alejandra

AU - Wurmser, Christine

AU - Perleberg, Carolin

AU - Pausch, Hubert

AU - Perkowska, Anna

AU - Fischer, Konrad

AU - Frishman, Dmitrij

AU - Fries, Ruedi

AU - Switonski, Marek

AU - Kind, Alexander

AU - Saur, Dieter

AU - Schnieke, Angelika

AU - Flisikowski, Krzysztof

PY - 2017/12/1

Y1 - 2017/12/1

N2 - We compared gene expression in low and high-grade intraepithelial dysplastic polyps from pigs carrying an APC 1311 truncating mutation orthologous to human APC 1309, analysing whole samples and microdissected dysplastic epithelium. Gene set enrichment analysis revealed differential expression of gene sets similar to human normal mucosa versus T1 stage polyps. Transcriptome analysis of whole samples revealed many differentially-expressed genes reflecting immune infiltration. Analysis of microdissected dysplastic epithelium was markedly different and showed increased expression in high-grade intraepithelial neoplasia of several genes known to be involved in human CRC; and revealed possible new roles for GBP6 and PLXND1. The pig model thus facilitates analysis of CRC pathogenesis.

AB - We compared gene expression in low and high-grade intraepithelial dysplastic polyps from pigs carrying an APC 1311 truncating mutation orthologous to human APC 1309, analysing whole samples and microdissected dysplastic epithelium. Gene set enrichment analysis revealed differential expression of gene sets similar to human normal mucosa versus T1 stage polyps. Transcriptome analysis of whole samples revealed many differentially-expressed genes reflecting immune infiltration. Analysis of microdissected dysplastic epithelium was markedly different and showed increased expression in high-grade intraepithelial neoplasia of several genes known to be involved in human CRC; and revealed possible new roles for GBP6 and PLXND1. The pig model thus facilitates analysis of CRC pathogenesis.

UR - https://www.scopus.com/pages/publications/85026368704

U2 - 10.1038/s41598-017-06741-8

DO - 10.1038/s41598-017-06741-8

M3 - Article

C2 - 28747659

AN - SCOPUS:85026368704

SN - 2045-2322

VL - 7

JO - Scientific Reports

JF - Scientific Reports

IS - 1

M1 - 6613

ER -

Porcine familial adenomatous polyposis model enables systematic analysis of early events in adenoma progression

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