TY - JOUR
T1 - Polynucleobacter necessarius, an obligate bacterial endosymbiont of the hypotrichous ciliate Euplotes aediculatus, is a member of the β-subclass of Proteobacteria
AU - Springer, Nina
AU - Amann, Rudolf
AU - Ludwig, Wolfgang
AU - Schleifer, Karl Heinz
AU - Schmidt, Helmut
PY - 1996/1/15
Y1 - 1996/1/15
N2 - An almost full length 16S rRNA gene of the obligate bacterial endosymbiont Polynucleobacter necessarius was amplified using the polymerase chain reaction in combination with site-specific primers. The amplified DNA was directly sequenced and compared with other bacterial 16S rRNA sequences. P. necessarius belongs to the β-subclass of Proteobacteria and shows the closest relationship to Alcaligenes eutrophus. Burkholderia solanacearum, and B. pickettii. In situ hybridization with a specific oligonucleotide probe corroborated the assignment of the retrieved sequence to P. necessarius.
AB - An almost full length 16S rRNA gene of the obligate bacterial endosymbiont Polynucleobacter necessarius was amplified using the polymerase chain reaction in combination with site-specific primers. The amplified DNA was directly sequenced and compared with other bacterial 16S rRNA sequences. P. necessarius belongs to the β-subclass of Proteobacteria and shows the closest relationship to Alcaligenes eutrophus. Burkholderia solanacearum, and B. pickettii. In situ hybridization with a specific oligonucleotide probe corroborated the assignment of the retrieved sequence to P. necessarius.
KW - 16S rRNA
KW - Bacterial endosymbiont
KW - Euplotes aediculatus
KW - In situ identification
KW - Phylogeny
KW - Polynucleobacter necessarius
UR - http://www.scopus.com/inward/record.url?scp=0030061074&partnerID=8YFLogxK
U2 - 10.1016/0378-1097(95)00476-9
DO - 10.1016/0378-1097(95)00476-9
M3 - Article
C2 - 8595875
AN - SCOPUS:0030061074
SN - 0378-1097
VL - 135
SP - 333
EP - 336
JO - FEMS Microbiology Letters
JF - FEMS Microbiology Letters
IS - 2-3
ER -