Polynucleobacter necessarius, an obligate bacterial endosymbiont of the hypotrichous ciliate Euplotes aediculatus, is a member of the β-subclass of Proteobacteria

Nina Springer, Rudolf Amann, Wolfgang Ludwig, Karl Heinz Schleifer, Helmut Schmidt

Research output: Contribution to journalArticlepeer-review

36 Scopus citations

Abstract

An almost full length 16S rRNA gene of the obligate bacterial endosymbiont Polynucleobacter necessarius was amplified using the polymerase chain reaction in combination with site-specific primers. The amplified DNA was directly sequenced and compared with other bacterial 16S rRNA sequences. P. necessarius belongs to the β-subclass of Proteobacteria and shows the closest relationship to Alcaligenes eutrophus. Burkholderia solanacearum, and B. pickettii. In situ hybridization with a specific oligonucleotide probe corroborated the assignment of the retrieved sequence to P. necessarius.

Original languageEnglish
Pages (from-to)333-336
Number of pages4
JournalFEMS Microbiology Letters
Volume135
Issue number2-3
DOIs
StatePublished - 15 Jan 1996
Externally publishedYes

Keywords

  • 16S rRNA
  • Bacterial endosymbiont
  • Euplotes aediculatus
  • In situ identification
  • Phylogeny
  • Polynucleobacter necessarius

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