TY - JOUR
T1 - Polymorphism in morels
T2 - Isozyme electrophoretic analysis
AU - Wipf, Daniel
AU - Bedell, Jean Philippe
AU - Munch, Jean Charles
AU - Botton, Bernard
AU - Buscot, François
PY - 1996
Y1 - 1996
N2 - The aim of this study was to assess whether isozyme polymorphism in different members of the Morchellaceae could be used to improve the systematics in this fungal group and to characterize intraspecific crossings between monosporal strains in Morchella esculenta. For this purpose, isozyme electrophoretic analysis of the following enzymes was performed: glutamine synthetase, NAD-glutamate dehydrogenase, NADP-glutamate dehydrogenase, aspartate aminotransferase, malate dehydrogenase, NAD-glyceraldehyde phosphate dehydrogenase, glucose phosphate isomerase, and superoxide dismutase. The analyses allowed discrimination at the inter- or intra-specific levels and could help to establish a method of identification for strains in the Morchellaceae. To a certain extent they appeared to be suitable to analyze interactions of monosporal strains of Morchella esculenta in pairing experiments. The polymorphism shown in this study was consistent with the phylogenetic relationships between the investigated strains only at the genus level.
AB - The aim of this study was to assess whether isozyme polymorphism in different members of the Morchellaceae could be used to improve the systematics in this fungal group and to characterize intraspecific crossings between monosporal strains in Morchella esculenta. For this purpose, isozyme electrophoretic analysis of the following enzymes was performed: glutamine synthetase, NAD-glutamate dehydrogenase, NADP-glutamate dehydrogenase, aspartate aminotransferase, malate dehydrogenase, NAD-glyceraldehyde phosphate dehydrogenase, glucose phosphate isomerase, and superoxide dismutase. The analyses allowed discrimination at the inter- or intra-specific levels and could help to establish a method of identification for strains in the Morchellaceae. To a certain extent they appeared to be suitable to analyze interactions of monosporal strains of Morchella esculenta in pairing experiments. The polymorphism shown in this study was consistent with the phylogenetic relationships between the investigated strains only at the genus level.
KW - Electrophoresis
KW - Isopzyme analysis
KW - Morchella sp.
KW - Polymorphism
UR - http://www.scopus.com/inward/record.url?scp=0029777438&partnerID=8YFLogxK
U2 - 10.1139/m96-103
DO - 10.1139/m96-103
M3 - Article
AN - SCOPUS:0029777438
SN - 0008-4166
VL - 42
SP - 819
EP - 827
JO - Canadian Journal of Microbiology
JF - Canadian Journal of Microbiology
IS - 8
ER -