Polymer-coated fluorescent CdSe-based quantum dots for application in immunoassay

Elena S. Speranskaya, Natalia V. Beloglazova, Pieterjan Lenain, Sarah De Saeger, Zhanhui Wang, Suxia Zhang, Zeger Hens, Dietmar Knopp, Reinhard Niessner, Dmitry V. Potapkin, Irina Yu Goryacheva

Research output: Contribution to journalArticlepeer-review

98 Scopus citations

Abstract

The paper describes all stages of synthesis and characterization of biocompatible CdSe-based core/shell quantum dots (QDs) and their application as fluorescent label for immunoassay. Special attention was focused on development of maleic anhydride-based amphiphilic polymers for QDs solubilization in aqueous media. In this work two PEG-amines were tried for polymer modification: monoamine Jeffamine M1000 used previously in some researches and diamine Jeffamine ED-2003 applied for the first time for QDs solubilization. The use of different Jeffamines allows us to obtain QDs with carboxyl or amine functional groups available for conjugation. The influence of polymer composition on optical properties of the nanocrystals and their stability in aqueous solutions as well as on their conjugation with biomolecules was studied. QDs with different coatings were used as biolabels in quantitative fluorescence microtiter plate immunoassay and qualitative on-site column test. It was found that quantum dots covered with amphiphilic polymer prepared from poly(maleic anhydride-alt-1-octadecene) and Jeffamine ED-2003 retained up to 90% of their initial brightness, easily conjugated with protein and showed low non-specific adsorption. In optimized conditions the obtained QDs were successfully used for determination of mycotoxin deoxynivalenol in wheat and maize samples by fluorescence microtiter plate immunoassay with an IC50 of 220μgkg-1 and by on-site column test with cut-off of 500μgkg-1.

Original languageEnglish
Pages (from-to)225-231
Number of pages7
JournalBiosensors and Bioelectronics
Volume53
DOIs
StatePublished - 2014

Keywords

  • Amphiphilic polymers
  • Deoxynivalenol
  • Fluorescent nanoparticles
  • Immunoassay
  • Quantum dots

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