TY - JOUR
T1 - Polimorfizm genu PRNP w kodonach 136, 154 i 171 u Polskich ras owiec
AU - Niznikowski, Roman
AU - Lühken, Gesine
AU - Strzelec, Ewa
AU - Lipsky, Shirin
AU - Popielarczyk, Dominik
AU - Erhardt, Georg
AU - Ajmone-Marsan, Paolo
AU - Bruford, Michael
AU - Dunner, Susana
AU - Erhardt, Georg
AU - Obexer-Ruff, Gabriela
AU - Taberlet, Pierre
AU - Valentini, Alessio
AU - Caloz, Régis
AU - Georgudis, Andreas
AU - Canali, Gabriele
AU - Roosen, Jutta
AU - Crepaldi, Paulo
AU - Togan, Inci
AU - Vlaic, Augustin
AU - Niznikowski, Roman
AU - Fésüs, László
AU - Ertugrul, Okan
AU - Abo-Shekada, Mohamoud
AU - Barody, Mohamed El
AU - Hoda, Anila
AU - Trommetter, Michel
PY - 2006/8
Y1 - 2006/8
N2 - The study was conducted on six Polish sheep breeds (Polish merino, Zelaznenska sheep, Pomeranian, Kamieniecka sheep, Polish mountain sheep and Polish heath sheep). Individual blood samples were collected and DNA was extracted. The frequencies of alleles (ARR, ARH, ARQ, AHQ, VRQ) and its genotypes of PRNP gene were determined according to the RFLP-PCR method. Depending on the breed and sex, a high diversity of polymorphous forms of the PRNP gene was observed. Higher PRNP gene polymorphism was observed in ewes than in rams. The lowest frequency of alleles was determined in three breeds: Polish merino (ARR, ARQ and VRQ), Polish mountain sheep and Polish heath sheep (ARR, ARQ and AHQ). All five of the determined alleles were observed in Pomeranian and Kamieniecka sheep. The most advantageous frequencies of ARR allele occurred in Polish merino, Kamieniecka and Zelaznenska sheep (particularly in ewes), in Polish mountain sheep and Polish heath sheep the VRQ allele was not observed. In the final results, 11 combinations of genotypes occurred in six Polish sheep breeds. The obtained results indicate a need for the construction of an appropriate breeding program for each breed separately. These breeding programs should enable obtaining higher frequencies of ARR allele occurrence and restricting the incidence of other alleles of the PRNP gene, particularly VRQ allele.
AB - The study was conducted on six Polish sheep breeds (Polish merino, Zelaznenska sheep, Pomeranian, Kamieniecka sheep, Polish mountain sheep and Polish heath sheep). Individual blood samples were collected and DNA was extracted. The frequencies of alleles (ARR, ARH, ARQ, AHQ, VRQ) and its genotypes of PRNP gene were determined according to the RFLP-PCR method. Depending on the breed and sex, a high diversity of polymorphous forms of the PRNP gene was observed. Higher PRNP gene polymorphism was observed in ewes than in rams. The lowest frequency of alleles was determined in three breeds: Polish merino (ARR, ARQ and VRQ), Polish mountain sheep and Polish heath sheep (ARR, ARQ and AHQ). All five of the determined alleles were observed in Pomeranian and Kamieniecka sheep. The most advantageous frequencies of ARR allele occurred in Polish merino, Kamieniecka and Zelaznenska sheep (particularly in ewes), in Polish mountain sheep and Polish heath sheep the VRQ allele was not observed. In the final results, 11 combinations of genotypes occurred in six Polish sheep breeds. The obtained results indicate a need for the construction of an appropriate breeding program for each breed separately. These breeding programs should enable obtaining higher frequencies of ARR allele occurrence and restricting the incidence of other alleles of the PRNP gene, particularly VRQ allele.
KW - PRNP
KW - Scrapie
KW - Sheep
UR - http://www.scopus.com/inward/record.url?scp=33747754523&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:33747754523
SN - 0025-8628
VL - 62
SP - 938
EP - 941
JO - Medycyna Weterynaryjna
JF - Medycyna Weterynaryjna
IS - 8
ER -