Plant regeneration from protoplasts of dessert banana cv. Grande Naine (Musa spp., Cavendish sub-group AAA) via somatic embryogenesis

A. Assani, R. Haicour, G. Wenzel, F. Côte, F. Bakry, B. Foroughi-Wehr, G. Ducreux, M. E. Aguillar, A. Grapin

Research output: Contribution to journalArticlepeer-review

50 Scopus citations

Abstract

Protoplast culture and plant regeneration of the dessert banana cultivar Grande Naine (Musa spp., Cavendish sub-group AAA) were achieved through somatic embryogenesis. Protoplasts were isolated from cell suspensions at a yield of 3×107 protoplasts/ml packed cell volume (0.5 g). For the induction of cell divisions, two banana cell suspensions, SF265 (AA) and IRFA903 (AA), were used as feeder layers. SF265 (AA) was found to be more efficient for inducing cell divisions than IRFA903 (AA). The first embryogenic cell suspensions were established from protoplast-derived microcalli. The transfer of microcalli and protoplast-derived cell suspensions onto regeneration medium containing plant growth regulators slightly increased the number of embryos relative to those maintained on a feeder layer with growth regulators. Plant regeneration was achieved in the same regeneration medium.

Original languageEnglish
Pages (from-to)482-488
Number of pages7
JournalPlant Cell Reports
Volume20
Issue number6
DOIs
StatePublished - 2001
Externally publishedYes

Keywords

  • Feeder cells
  • Musa spp.
  • Plant regeneration
  • Protoplast culture
  • Somatic embryos

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