Pituitary ATP receptors: Characterization and functional localization to gonadotropes

Zhen Ping Chen, Andrew Levy, Craig A. McArdle, Stafford L. Lightman

Research output: Contribution to journalArticlepeer-review

37 Scopus citations

Abstract

We have used real-time dynamic video imaging of Fura-2 fluorescence to study the acute effects of extracellular ATP on [Ca2+](i) in pituitary cells and found that ATP produced a rapid biphasic cytosolic Ca2+ increase in a dose-dependent manner in both rat pituitary gonadotropes and gonadotrope-derived αT3-1 cells. Removal of extracellular Ca2+ only attenuated the plateau phase of ATP-induced intracellular Ca2+ response and suramin (100 μM), a P2-purinoceptor antagonist, reduced the ATP effect by 40 - 60 %. Further studies revealed the following rank-order of agonist potency in the Ca2+ response: ATP = ADP = UTP > 2-methylthioATP >> β,γ- methylene ATP/α,β-methylene ATP. Both homologous and heterologous desensitization occurred with ATP and UTP and these did not have additive effects. Together the data suggest that the intracellular Ca2+ responses of gonadotropes and αT3-1 cells to these nucleotides are mediated by a single class of receptor pharmacologically characterized as the P(2U) subtype. This is the first demonstration of functional ATP receptors in pituitary gonadotropes.

Original languageEnglish
Pages (from-to)1280-1283
Number of pages4
JournalEndocrinology
Volume135
Issue number3
DOIs
StatePublished - Sep 1994
Externally publishedYes

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