Phytochelatins are synthesized by two vacuolar serine carboxypeptidases in Saccharomyces cerevisiae

Jana Wünschmann; Andreas Beck; Laurent Meyer; Thomas Letzel; Erwin Grill; Klaus J. Lendzian

doi:10.1016/j.febslet.2007.03.039

Phytochelatins are synthesized by two vacuolar serine carboxypeptidases in Saccharomyces cerevisiae

Jana Wünschmann, Andreas Beck, Laurent Meyer, Thomas Letzel, Erwin Grill, Klaus J. Lendzian

Technical University of Munich

Research output: Contribution to journal › Article › peer-review

30 Scopus citations

Abstract

Phytochelatins (PCs) are cysteine-rich peptides that chelate heavy metal ions, thereby mediating heavy metal tolerance in plants, fission yeast, and Caenorhabditis elegans. They are synthesized from glutathione by PC synthase, a specific dipeptidyltransferase. While Saccharomyces cerevisiae synthesizes PCs upon exposure to heavy metal ions, the S. cerevisiae genome does not encode a PC synthase homologue. How PCs are synthesized in yeast is unclear. This study shows that the vacuolar serine carboxypeptidases CPY and CPC are responsible for PC synthesis in yeast. The finding of a PCS-like activity of these enzymes in vivo discloses another route for PC biosynthesis in eukaryotes.

Original language	English
Pages (from-to)	1681-1687
Number of pages	7
Journal	FEBS Letters
Volume	581
Issue number	8
DOIs	https://doi.org/10.1016/j.febslet.2007.03.039
State	Published - 17 Apr 2007

Keywords

Carboxypeptidase C
Carboxypeptidase Y
Glutathione
Metal tolerance
S. cerevisiae

Access to Document

10.1016/j.febslet.2007.03.039

Cite this

@article{4e7f50d6eab545a1bca6fc3d3c6ae7f5,

title = "Phytochelatins are synthesized by two vacuolar serine carboxypeptidases in Saccharomyces cerevisiae",

abstract = "Phytochelatins (PCs) are cysteine-rich peptides that chelate heavy metal ions, thereby mediating heavy metal tolerance in plants, fission yeast, and Caenorhabditis elegans. They are synthesized from glutathione by PC synthase, a specific dipeptidyltransferase. While Saccharomyces cerevisiae synthesizes PCs upon exposure to heavy metal ions, the S. cerevisiae genome does not encode a PC synthase homologue. How PCs are synthesized in yeast is unclear. This study shows that the vacuolar serine carboxypeptidases CPY and CPC are responsible for PC synthesis in yeast. The finding of a PCS-like activity of these enzymes in vivo discloses another route for PC biosynthesis in eukaryotes.",

keywords = "Carboxypeptidase C, Carboxypeptidase Y, Glutathione, Metal tolerance, S. cerevisiae",

author = "Jana W{\"u}nschmann and Andreas Beck and Laurent Meyer and Thomas Letzel and Erwin Grill and Lendzian, {Klaus J.}",

note = "Funding Information: The financial support of the Deutsche Forschungsgemeinschaft and the Fonds der Chemischen Industrie is gratefully acknowledged. We are grateful to Johanna Berger and Sylvia Gro{\ss}e for technical assistance and to Dr. Farhah Assaad for critical reading of the manuscript. Thomas Letzel thanks Agilent (Waldbronn, Germany) for providing the LC-quadrupole-MS.",

year = "2007",

month = apr,

day = "17",

doi = "10.1016/j.febslet.2007.03.039",

language = "English",

volume = "581",

pages = "1681--1687",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "John Wiley and Sons Inc.",

number = "8",

}

TY - JOUR

T1 - Phytochelatins are synthesized by two vacuolar serine carboxypeptidases in Saccharomyces cerevisiae

AU - Wünschmann, Jana

AU - Beck, Andreas

AU - Meyer, Laurent

AU - Letzel, Thomas

AU - Grill, Erwin

AU - Lendzian, Klaus J.

N1 - Funding Information: The financial support of the Deutsche Forschungsgemeinschaft and the Fonds der Chemischen Industrie is gratefully acknowledged. We are grateful to Johanna Berger and Sylvia Große for technical assistance and to Dr. Farhah Assaad for critical reading of the manuscript. Thomas Letzel thanks Agilent (Waldbronn, Germany) for providing the LC-quadrupole-MS.

PY - 2007/4/17

Y1 - 2007/4/17

N2 - Phytochelatins (PCs) are cysteine-rich peptides that chelate heavy metal ions, thereby mediating heavy metal tolerance in plants, fission yeast, and Caenorhabditis elegans. They are synthesized from glutathione by PC synthase, a specific dipeptidyltransferase. While Saccharomyces cerevisiae synthesizes PCs upon exposure to heavy metal ions, the S. cerevisiae genome does not encode a PC synthase homologue. How PCs are synthesized in yeast is unclear. This study shows that the vacuolar serine carboxypeptidases CPY and CPC are responsible for PC synthesis in yeast. The finding of a PCS-like activity of these enzymes in vivo discloses another route for PC biosynthesis in eukaryotes.

AB - Phytochelatins (PCs) are cysteine-rich peptides that chelate heavy metal ions, thereby mediating heavy metal tolerance in plants, fission yeast, and Caenorhabditis elegans. They are synthesized from glutathione by PC synthase, a specific dipeptidyltransferase. While Saccharomyces cerevisiae synthesizes PCs upon exposure to heavy metal ions, the S. cerevisiae genome does not encode a PC synthase homologue. How PCs are synthesized in yeast is unclear. This study shows that the vacuolar serine carboxypeptidases CPY and CPC are responsible for PC synthesis in yeast. The finding of a PCS-like activity of these enzymes in vivo discloses another route for PC biosynthesis in eukaryotes.

KW - Carboxypeptidase C

KW - Carboxypeptidase Y

KW - Glutathione

KW - Metal tolerance

KW - S. cerevisiae

UR - http://www.scopus.com/inward/record.url?scp=34047142681&partnerID=8YFLogxK

U2 - 10.1016/j.febslet.2007.03.039

DO - 10.1016/j.febslet.2007.03.039

M3 - Article

C2 - 17408619

AN - SCOPUS:34047142681

SN - 0014-5793

VL - 581

SP - 1681

EP - 1687

JO - FEBS Letters

JF - FEBS Letters

IS - 8

ER -

Phytochelatins are synthesized by two vacuolar serine carboxypeptidases in Saccharomyces cerevisiae

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this