Phenolic compounds of Iris adriatica and their antimycobacterial effects

Sandra Ochensberger; Fabian Alperth; Božena Mitić; Olaf Kunert; Stefanie Mayer; Maria Ferreira Mourão; Ivana Turek; Simon Vlad Luca; Krystyna Skalicka-Woźniak; Željan Maleš; Dario Hruševar; Ivan Duka; Franz Bucar

doi:10.2478/acph-2019-0037

Phenolic compounds of Iris adriatica and their antimycobacterial effects

Sandra Ochensberger
, Fabian Alperth
, Božena Mitić
, Olaf Kunert
, Stefanie Mayer
, Maria Ferreira Mourão
, Ivana Turek
, Simon Vlad Luca
, Krystyna Skalicka-Woźniak
, Željan Maleš
, Dario Hruševar
, Ivan Duka
, Franz Bucar

University of Graz
University of Zagreb
ISEG-Technical University of Lisbon
“Grigore T. Popa” University of Medicine and Pharmacy Iasi
Medical University of Lublin
University of Zagreb

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Little is known about the pharmacological activities of Iris adriatica (Iridaceae), a plant endemic to Dalmatia (Croatia). We therefore performed a bioassay-guided fractionation including high-performance counter current chromatography (HPCCC) and antibacterial tests using Mycobacterium smegmatis mc2 155. One obtained fraction was found to be antimycobacterially active with a MIC of 64 mg L-1. Furthermore, fractions were tested for resistance modulatory effects using ethidium bromide as substrate. We were able to identify the pure isoflavonic compounds irigenin and irilone and a fraction containing mainly benzophenone 2,4,6-trihydroxy-4-methoxy-benzophenone, responsible for the resistance-modulatory activity of this plant.

Original language	English
Pages (from-to)	673-681
Number of pages	9
Journal	Acta Pharmaceutica
Volume	69
Issue number	4
DOIs	https://doi.org/10.2478/acph-2019-0037
State	Published - 1 Dec 2019
Externally published	Yes

Keywords

Iris adriatica
MIC
Mycobacterium smegmatis
Radix Iridis
ethidium bromide
phenolic compounds
resistance modulation

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10.2478/acph-2019-0037

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title = "Phenolic compounds of Iris adriatica and their antimycobacterial effects",

abstract = "Little is known about the pharmacological activities of Iris adriatica (Iridaceae), a plant endemic to Dalmatia (Croatia). We therefore performed a bioassay-guided fractionation including high-performance counter current chromatography (HPCCC) and antibacterial tests using Mycobacterium smegmatis mc2 155. One obtained fraction was found to be antimycobacterially active with a MIC of 64 mg L-1. Furthermore, fractions were tested for resistance modulatory effects using ethidium bromide as substrate. We were able to identify the pure isoflavonic compounds irigenin and irilone and a fraction containing mainly benzophenone 2,4,6-trihydroxy-4-methoxy-benzophenone, responsible for the resistance-modulatory activity of this plant.",

keywords = "Iris adriatica, MIC, Mycobacterium smegmatis, Radix Iridis, ethidium bromide, phenolic compounds, resistance modulation",

author = "Sandra Ochensberger and Fabian Alperth and Bo{\v z}ena Miti{\'c} and Olaf Kunert and Stefanie Mayer and Mour{\~a}o, \{Maria Ferreira\} and Ivana Turek and Luca, \{Simon Vlad\} and Krystyna Skalicka-Wo{\'z}niak and {\v Z}eljan Male{\v s} and Dario Hru{\v s}evar and Ivan Duka and Franz Bucar",

year = "2019",

month = dec,

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doi = "10.2478/acph-2019-0037",

language = "English",

volume = "69",

pages = "673--681",

journal = "Acta Pharmaceutica",

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T1 - Phenolic compounds of Iris adriatica and their antimycobacterial effects

AU - Ochensberger, Sandra

AU - Alperth, Fabian

AU - Mitić, Božena

AU - Kunert, Olaf

AU - Mayer, Stefanie

AU - Mourão, Maria Ferreira

AU - Turek, Ivana

AU - Luca, Simon Vlad

AU - Skalicka-Woźniak, Krystyna

AU - Maleš, Željan

AU - Hruševar, Dario

AU - Duka, Ivan

AU - Bucar, Franz

PY - 2019/12/1

Y1 - 2019/12/1

N2 - Little is known about the pharmacological activities of Iris adriatica (Iridaceae), a plant endemic to Dalmatia (Croatia). We therefore performed a bioassay-guided fractionation including high-performance counter current chromatography (HPCCC) and antibacterial tests using Mycobacterium smegmatis mc2 155. One obtained fraction was found to be antimycobacterially active with a MIC of 64 mg L-1. Furthermore, fractions were tested for resistance modulatory effects using ethidium bromide as substrate. We were able to identify the pure isoflavonic compounds irigenin and irilone and a fraction containing mainly benzophenone 2,4,6-trihydroxy-4-methoxy-benzophenone, responsible for the resistance-modulatory activity of this plant.

AB - Little is known about the pharmacological activities of Iris adriatica (Iridaceae), a plant endemic to Dalmatia (Croatia). We therefore performed a bioassay-guided fractionation including high-performance counter current chromatography (HPCCC) and antibacterial tests using Mycobacterium smegmatis mc2 155. One obtained fraction was found to be antimycobacterially active with a MIC of 64 mg L-1. Furthermore, fractions were tested for resistance modulatory effects using ethidium bromide as substrate. We were able to identify the pure isoflavonic compounds irigenin and irilone and a fraction containing mainly benzophenone 2,4,6-trihydroxy-4-methoxy-benzophenone, responsible for the resistance-modulatory activity of this plant.

KW - Iris adriatica

KW - MIC

KW - Mycobacterium smegmatis

KW - Radix Iridis

KW - ethidium bromide

KW - phenolic compounds

KW - resistance modulation

UR - https://www.scopus.com/pages/publications/85073721926

U2 - 10.2478/acph-2019-0037

DO - 10.2478/acph-2019-0037

M3 - Article

C2 - 31639092

AN - SCOPUS:85073721926

SN - 1330-0075

VL - 69

SP - 673

EP - 681

JO - Acta Pharmaceutica

JF - Acta Pharmaceutica

IS - 4

ER -

Phenolic compounds of Iris adriatica and their antimycobacterial effects

Abstract

Keywords

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