Perspectives for sensitivity enhancement in proton-detected solid-state NMR of highly deuterated proteins by preserving water magnetization

Veniamin Chevelkov, Shengqi Xiang, Karin Giller, Stefan Becker, Adam Lange, Bernd Reif

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

In this work, we show how the water flip-back approach that is widely employed in solution-state NMR can be adapted to proton-detected MAS solid-state NMR of highly deuterated proteins. The scheme allows to enhance the sensitivity of the experiment by decreasing the recovery time of the proton longitudinal magnetization. The method relies on polarization transfer from non-saturated water to the protein during the inter-scan delay.

Original languageEnglish
Pages (from-to)151-160
Number of pages10
JournalJournal of Biomolecular NMR
Volume61
Issue number2
DOIs
StatePublished - Feb 2015

Keywords

  • Magic angle spinning (MAS) solid-state NMR
  • Perdeuteration
  • Protein water interaction
  • Sensitivity enchancement

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