Persistence of a yeast-based (Hanseniaspora uvarum) attract-and-kill formulation against drosophila Suzukii on grape leaves

Flavia Bianchi, Urban Spitaler, Irene Castellan, Carlo S. Cossu, Timothy Brigadoi, Claire Duménil, Sergio Angeli, Peter Robatscher, Rudi F. Vogel, Silvia Schmidt, Daniela Eisenstecken

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

The production of phagostimulant and attractive volatile organic compounds (VOCs) by yeasts can be exploited to improve the efficacy of attract-and-kill formulations against the spotted wing drosophila (SWD). This study evaluated the persistence over one week of a yeast-based formulation under greenhouse conditions. Potted grape plants were treated with: (i) potato dextrose broth (PDB), (ii) PDB containing spinosad (PDB + S), and (iii) H. uvarum fermentation broth grown on PDB containing spinosad (H. u. + S). Laboratory trials were performed to determine the survival and the oviposition rate of SWD after exposure to treated leaves. Ion-exchange chromatography was performed to measure carbohydrates, sugar alcohols, and organic acids on leaf surfaces, while amino acids were assessed through liquid chromatography–mass-spectrometry. Additionally, the VOCs released by plants treated with H.uvarum were collected via closed-loop-stripping analysis and compared to those emitted by untreated leaves. A higher mortality was observed for adult SWDs in contact with H. uvarum containing spinosad compared to PDB containing spinosad. Generally, a decrease in the amounts of non-volatile compounds was observed over time, though numerous nutrients were still present one week after treatment. The application of the yeast-based formulation induced the emission of VOCs by the treated leaves. The concentration of 2-phenylethanol, one of the main VOCs emitted by yeasts, decreased over time. These findings describe the presence of potential phagostimulants and compounds attractive to SWD in a yeast-based attract-and-kill formulation and demonstrate the efficacy of the formulation over one week.

Original languageEnglish
Article number810
Pages (from-to)1-17
Number of pages17
JournalInsects
Volume11
Issue number11
DOIs
StatePublished - Nov 2020
Externally publishedYes

Keywords

  • Metabolites
  • Pest control
  • Spinosad
  • Spotted wing drosophila
  • VOCs

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