Peptide mapping and characterisation of glycation patterns of the glima 38 antigen recognised by autoantibodies in Type I diabetic patients

U. Roll, C. W. Turck, S. E. Gitelman, S. M. Rosenthal, M. S. Nolte, U. Masharani, A. G. Ziegler, S. Baekkeskov

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Aims/hypothesis. Glima 38 is an N-glycated neuroendocrine membrane protein of M(r) 38 000, which is recognised by autoantibodies in approximately 20% of patients with Type I (insulin-dependent) diabetes mellitus. The aim of this study was to characterise the carbohydrate moiety and generate peptide maps of glima 38. Methods. Sera of high immunoreactivity to glima 38 were used to isolate 35-S methionine-labelled protein from βTC-3 cells and a neuronal cell line GT1.7. Tunicamycin was used to inhibit N- glycation of glima 38 and define the core protein. The carbohydrate moiety was characterised for tunicamycin sensitivity, lectin binding and susceptibility to different endoglycosidases. The protein moiety was subjected to digestion by proteases to define peptide maps. Results. The autoreactive epitopes in glima 38 recognised by Type I diabetic sera are conformational and independent of the carbohydrate moiety. Inhibition of N- glycation of glima 38 in vivo, shows a protein core of M(r) 22 000 in both pancreatic β-(βTC3) and neuronal (GT1.7) cell lines. The carbohydrate moieties in the two cell types are distinct but contain a similar amount of terminal sialic acid residues and at least five oligosaccharide chains Glima 38 binds Triticum vulgare and Ricinus communis I lectins. Endoproteinase treatment of the M(r) 22 000 core protein results in peptides of M(r) 4500 and M(r) 20 000 with Lys-C, and peptides of M(r) 4 000 and M(r) 11 000-12000 with Glu-C/V8 and Asp-N proteases. Conclusion/interpretation. The biochemical properties of glima 38 define it as a new autoantigen in Type I diabetes and provide a basis for its purification.

Original languageEnglish
Pages (from-to)598-608
Number of pages11
JournalDiabetologia
Volume43
Issue number5
DOIs
StatePublished - 2000
Externally publishedYes

Keywords

  • 38 000 M(r) autoantigen
  • Autoantibodies
  • Deglycation
  • Glima 38
  • Immunology
  • Lectin binding
  • Peptide mapping
  • Proteolytic cleavage
  • Target autoantigen
  • Type I diabetes

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