PARP1: A Potential Molecular Marker to Identify Cancer During Colposcopy Procedures

Paula Demétrio de Souza França, Navjot Guru, Abigail R. Kostolansky, Audrey Mauguen, Giacomo Pirovano, Susanne Kossatz, Sheryl Roberts, Marcio Abrahão, Snehal G. Patel, Kay J. Park, Thomas Reiner, Elizabeth Jewell

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Despite efforts in prevention, cervical cancer still presents with a high worldwide incidence and remains a great problem in public health, especially in low-income countries. Screening programs, such as colposcopy with Papanicolaou testing, have greatly improved mortality rates. However, the agents currently used to delineate those lesions (topical application of acetic acid or Lugol iodine) lack specificity and sometimes can lead to unnecessary biopsies or even cervical excisions. A tool to enable in vivo histology to quickly and quantitatively distinguish between tumor, dysplastic tissue, and healthy tissue would be of great clinical interest. Methods: Here, we describe the use of PARPi-FL, a fluorescent inhibitor of poly[adenosine diphosphateribose] polymerase 1 (PARP1), which is a nuclear enzyme that is overexpressed in cancer when compared with the normal surrounding tissues. We exploit its use as an optical imaging agent to specifically target PARP1 expression, which was demonstrated to be higher in cervical cancer than the normal surrounding tissue. Results: After topical application of PARPi-FL on freshly excised cone biopsy samples, the nuclei of tumor cells emitted a specific fluorescent signal that could be visualized using a handheld fluorescence confocal microscope. Conclusion: This approach has the potential to improve in vivo identification of tumor cells during colposcopy examination, allowing a rapid, noninvasive, and accurate histopathologic assessment.

Original languageEnglish
Pages (from-to)941-948
Number of pages8
JournalJournal of Nuclear Medicine
Volume62
Issue number7
DOIs
StatePublished - 1 Jul 2021
Externally publishedYes

Keywords

  • PARP1
  • PARPi-FL
  • cervical cancer
  • fluorescence-guided surgery

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