Parameterization of hyperpolarized 13C-bicarbonate-dissolution dynamic nuclear polarization

David Johannes Scholz; Angela M. Otto; Josef Hintermair; Franz Schilling; Annette Frank; Ulrich Köllisch; Martin A. Janich; Rolf F. Schulte; Markus Schwaiger; Axel Haase; Marion I. Menzel

doi:10.1007/s10334-015-0500-9

Parameterization of hyperpolarized ¹³C-bicarbonate-dissolution dynamic nuclear polarization

David Johannes Scholz
, Angela M. Otto
, Josef Hintermair
, Franz Schilling
, Annette Frank
, Ulrich Köllisch
, Martin A. Janich
, Rolf F. Schulte
, Markus Schwaiger
, Axel Haase
, Marion I. Menzel

Technical University of Munich
GE Global Research - Europe

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Objective: ¹³C metabolic MRI using hyperpolarized ¹³C-bicarbonate enables preclinical detection of pH. To improve signal-to-noise ratio, experimental procedures were refined, and the influence of pH, buffer capacity, temperature, and field strength were investigated. Materials and methods: Bicarbonate preparation was investigated. Bicarbonate was prepared and applied in spectroscopy at 1, 3, 14 T using pure dissolution, culture medium, and MCF-7 cell spheroids. Healthy rats were imaged by spectral–spatial spiral acquisition for spatial and temporal bicarbonate distribution, pH mapping, and signal decay analysis. Results: An optimized preparation technique for maximum solubility of 6 mol/L and polarization levels of 19–21 % is presented; T₁ and SNR dependency on field strength, buffer capacity, and pH was investigated. pH mapping in vivo is demonstrated. Conclusion: An optimized bicarbonate preparation and experimental procedure provided improved T₁ and SNR values, allowing in vitro and in vivo applications.

Original language	English
Pages (from-to)	591-598
Number of pages	8
Journal	Magnetic Resonance Materials in Physics, Biology and Medicine
Volume	28
Issue number	6
DOIs	https://doi.org/10.1007/s10334-015-0500-9
State	Published - 1 Dec 2015

Keywords

Cell spheroids
Hyperpolarized C
MCF-7
NMR spectroscopy

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10.1007/s10334-015-0500-9

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Scholz, D. J., Otto, A. M., Hintermair, J., Schilling, F., Frank, A., Köllisch, U., Janich, M. A., Schulte, R. F., Schwaiger, M., Haase, A., & Menzel, M. I. (2015). Parameterization of hyperpolarized ¹³C-bicarbonate-dissolution dynamic nuclear polarization. Magnetic Resonance Materials in Physics, Biology and Medicine, 28(6), 591-598. https://doi.org/10.1007/s10334-015-0500-9

@article{0b1aeb4635554703b88164b38b7cad6b,

title = "Parameterization of hyperpolarized 13C-bicarbonate-dissolution dynamic nuclear polarization",

abstract = "Objective: 13C metabolic MRI using hyperpolarized 13C-bicarbonate enables preclinical detection of pH. To improve signal-to-noise ratio, experimental procedures were refined, and the influence of pH, buffer capacity, temperature, and field strength were investigated. Materials and methods: Bicarbonate preparation was investigated. Bicarbonate was prepared and applied in spectroscopy at 1, 3, 14 T using pure dissolution, culture medium, and MCF-7 cell spheroids. Healthy rats were imaged by spectral–spatial spiral acquisition for spatial and temporal bicarbonate distribution, pH mapping, and signal decay analysis. Results: An optimized preparation technique for maximum solubility of 6 mol/L and polarization levels of 19–21 \% is presented; T1 and SNR dependency on field strength, buffer capacity, and pH was investigated. pH mapping in vivo is demonstrated. Conclusion: An optimized bicarbonate preparation and experimental procedure provided improved T1 and SNR values, allowing in vitro and in vivo applications.",

keywords = "Cell spheroids, Hyperpolarized C, MCF-7, NMR spectroscopy",

author = "Scholz, \{David Johannes\} and Otto, \{Angela M.\} and Josef Hintermair and Franz Schilling and Annette Frank and Ulrich K{\"o}llisch and Janich, \{Martin A.\} and Schulte, \{Rolf F.\} and Markus Schwaiger and Axel Haase and Menzel, \{Marion I.\}",

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year = "2015",

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doi = "10.1007/s10334-015-0500-9",

language = "English",

volume = "28",

pages = "591--598",

journal = "Magnetic Resonance Materials in Physics, Biology and Medicine",

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Scholz, DJ, Otto, AM, Hintermair, J, Schilling, F, Frank, A, Köllisch, U, Janich, MA, Schulte, RF, Schwaiger, M, Haase, A & Menzel, MI 2015, 'Parameterization of hyperpolarized ¹³C-bicarbonate-dissolution dynamic nuclear polarization', Magnetic Resonance Materials in Physics, Biology and Medicine, vol. 28, no. 6, pp. 591-598. https://doi.org/10.1007/s10334-015-0500-9

TY - JOUR

T1 - Parameterization of hyperpolarized 13C-bicarbonate-dissolution dynamic nuclear polarization

AU - Scholz, David Johannes

AU - Otto, Angela M.

AU - Hintermair, Josef

AU - Schilling, Franz

AU - Frank, Annette

AU - Köllisch, Ulrich

AU - Janich, Martin A.

AU - Schulte, Rolf F.

AU - Schwaiger, Markus

AU - Haase, Axel

AU - Menzel, Marion I.

PY - 2015/12/1

Y1 - 2015/12/1

N2 - Objective: 13C metabolic MRI using hyperpolarized 13C-bicarbonate enables preclinical detection of pH. To improve signal-to-noise ratio, experimental procedures were refined, and the influence of pH, buffer capacity, temperature, and field strength were investigated. Materials and methods: Bicarbonate preparation was investigated. Bicarbonate was prepared and applied in spectroscopy at 1, 3, 14 T using pure dissolution, culture medium, and MCF-7 cell spheroids. Healthy rats were imaged by spectral–spatial spiral acquisition for spatial and temporal bicarbonate distribution, pH mapping, and signal decay analysis. Results: An optimized preparation technique for maximum solubility of 6 mol/L and polarization levels of 19–21 % is presented; T1 and SNR dependency on field strength, buffer capacity, and pH was investigated. pH mapping in vivo is demonstrated. Conclusion: An optimized bicarbonate preparation and experimental procedure provided improved T1 and SNR values, allowing in vitro and in vivo applications.

AB - Objective: 13C metabolic MRI using hyperpolarized 13C-bicarbonate enables preclinical detection of pH. To improve signal-to-noise ratio, experimental procedures were refined, and the influence of pH, buffer capacity, temperature, and field strength were investigated. Materials and methods: Bicarbonate preparation was investigated. Bicarbonate was prepared and applied in spectroscopy at 1, 3, 14 T using pure dissolution, culture medium, and MCF-7 cell spheroids. Healthy rats were imaged by spectral–spatial spiral acquisition for spatial and temporal bicarbonate distribution, pH mapping, and signal decay analysis. Results: An optimized preparation technique for maximum solubility of 6 mol/L and polarization levels of 19–21 % is presented; T1 and SNR dependency on field strength, buffer capacity, and pH was investigated. pH mapping in vivo is demonstrated. Conclusion: An optimized bicarbonate preparation and experimental procedure provided improved T1 and SNR values, allowing in vitro and in vivo applications.

KW - Cell spheroids

KW - Hyperpolarized C

KW - MCF-7

KW - NMR spectroscopy

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U2 - 10.1007/s10334-015-0500-9

DO - 10.1007/s10334-015-0500-9

M3 - Article

C2 - 26449715

AN - SCOPUS:84947612721

SN - 0968-5243

VL - 28

SP - 591

EP - 598

JO - Magnetic Resonance Materials in Physics, Biology and Medicine

JF - Magnetic Resonance Materials in Physics, Biology and Medicine

IS - 6

ER -

Parameterization of hyperpolarized ¹³C-bicarbonate-dissolution dynamic nuclear polarization

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