TY - JOUR
T1 - Oxygen utilization by Lactobacillus plantarum - II. Superoxide and superoxide dismutation
AU - Götz, F.
AU - Elstner, E. F.
AU - Sedewitz, B.
AU - Lengfelder, E.
PY - 1980/4
Y1 - 1980/4
N2 - Cell-free extracts of Lactobacillus plantarum contain non-proteinaceous compounds which mimic superoxide dismutase activity. Using the test system in which O2-is generated by xanthine oxidase, superoxide dismutase activity is found in cell-free extracts, where proteins are removed by precipitation. This activity is strongly decreased after dialysis of cell-free extracts. Superoxide dismutase activity was also investigated by means of pulse radiolysis. Cell-free extracts of Escherichia coli were also investigated as a comparison, which were known to contain superoxide dismutase. With cell-free extracts of both L. plantarum and E. coli the decay of O2-was markedly increased. However, the type of reaction of the O2-decay was of first order in the presence of E. coli extracts due to superoxide dismutase(s), and of second order in the presence of L. plantarum extracts, indicating that O2-elimination is not an enzymic reaction. Mn2+ phosphate(s) might be responsible for the observed elimination of O2-. The production of O2-is not detectable during NADH-, lactate- or pyruvate oxidase reactions in L. plantarum extracts.
AB - Cell-free extracts of Lactobacillus plantarum contain non-proteinaceous compounds which mimic superoxide dismutase activity. Using the test system in which O2-is generated by xanthine oxidase, superoxide dismutase activity is found in cell-free extracts, where proteins are removed by precipitation. This activity is strongly decreased after dialysis of cell-free extracts. Superoxide dismutase activity was also investigated by means of pulse radiolysis. Cell-free extracts of Escherichia coli were also investigated as a comparison, which were known to contain superoxide dismutase. With cell-free extracts of both L. plantarum and E. coli the decay of O2-was markedly increased. However, the type of reaction of the O2-decay was of first order in the presence of E. coli extracts due to superoxide dismutase(s), and of second order in the presence of L. plantarum extracts, indicating that O2-elimination is not an enzymic reaction. Mn2+ phosphate(s) might be responsible for the observed elimination of O2-. The production of O2-is not detectable during NADH-, lactate- or pyruvate oxidase reactions in L. plantarum extracts.
KW - Lactobacillus plantarum
KW - Pulse radiolysis
KW - Superoxide anion
KW - Superoxide dismutase
UR - http://www.scopus.com/inward/record.url?scp=0019303319&partnerID=8YFLogxK
U2 - 10.1007/BF00446879
DO - 10.1007/BF00446879
M3 - Article
C2 - 6246845
AN - SCOPUS:0019303319
SN - 0302-8933
VL - 125
SP - 215
EP - 220
JO - Archives of Microbiology
JF - Archives of Microbiology
IS - 3
ER -