Oxygen supply maps for hypoxic microenvironment visualization in prostate cancer

Niels J. Rupp; Peter J. Schuffler; Qing Zhong; Florian Falkner; Markus Rechsteiner; Jan H. Ruschoff; Christian Fankhauser; Matthias Drach; Remo Largo; Mathias Tremp; Cedric Poyet; Tullio Sulser; Glen Kristiansen; Holger Moch; Joachim Buhmann; Michael Muntener; Peter Wild

doi:10.4103/2153-3539.175376

Oxygen supply maps for hypoxic microenvironment visualization in prostate cancer

Niels J. Rupp
, Peter J. Schuffler
, Qing Zhong
, Florian Falkner
, Markus Rechsteiner
, Jan H. Ruschoff
, Christian Fankhauser
, Matthias Drach
, Remo Largo
, Mathias Tremp
, Cedric Poyet
, Tullio Sulser
, Glen Kristiansen
, Holger Moch
, Joachim Buhmann
, Michael Muntener
, Peter Wild

University Hospital Zurich
ETH Zürich
Cantonal Hospital Winterthur
University Hospital Basel
University of Bonn and University Hospital Bonn
City Hospital Triemli

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

Background: Intratumoral hypoxia plays an important role with regard to tumor biology and susceptibility to radio. and chemotherapy. For further investigation of hypoxia.related changes, areas of certain hypoxia must be reliably detected within cancer tissues. Pimonidazole, a 2.nitroimindazole, accumulates in hypoxic tissue and can be easily visualized using immunohistochemistry. Materials and Methods: To improve detection of highly hypoxic versus normoxic areas in prostate cancer, immunoreactivity of pimonidazole and a combination of known hypoxia.related proteins was used to create computational oxygen supply maps of prostate cancer. Pimonidazole was intravenously administered before radical prostatectomy in n = 15 patients, using the da Vinci robot.assisted surgical system. Prostatectomy specimens were immediately transferred into buffered formaldehyde, fixed overnight, and completely embedded in paraffin. Pimonidazole accumulation and hypoxia.related protein expression were visualized by immunohistochemistry. Oxygen supply maps were created using the normalized information from pimonidazole and hypoxia.related proteins. Results: Based on pimonidazole staining and other hypoxia.related proteins (osteopontin, hypoxia.inducible factor 1.alpha, and glucose transporter member 1) oxygen supply maps in prostate cancer were created. Overall, oxygen supply maps consisting of information from all hypoxia.related proteins showed high correlation and mutual information to the golden standard of pimonidazole. Here, we describe an improved computer.based ex vivo model for an accurate detection of oxygen supply in human prostate cancer tissue. Conclusions: This platform can be used for precise colocalization of novel candidate hypoxia.related proteins in a representative number of prostate cancer cases, and improve issues of single marker correlations. Furthermore, this study provides a source for further in situ tests and biochemical investigations.

Original language	English
Article number	3
Journal	Journal of Pathology Informatics
Volume	7
Issue number	1
DOIs	https://doi.org/10.4103/2153-3539.175376
State	Published - 29 Jan 2016
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Hypoxia
pimonidazole
prostate cancer

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10.4103/2153-3539.175376

Cite this

Rupp, N. J., Schuffler, P. J., Zhong, Q., Falkner, F., Rechsteiner, M., Ruschoff, J. H., Fankhauser, C., Drach, M., Largo, R., Tremp, M., Poyet, C., Sulser, T., Kristiansen, G., Moch, H., Buhmann, J., Muntener, M., & Wild, P. (2016). Oxygen supply maps for hypoxic microenvironment visualization in prostate cancer. Journal of Pathology Informatics, 7(1), Article 3. https://doi.org/10.4103/2153-3539.175376

@article{4f404e5577e14d8caeb3b2670211c8ce,

title = "Oxygen supply maps for hypoxic microenvironment visualization in prostate cancer",

abstract = "Background: Intratumoral hypoxia plays an important role with regard to tumor biology and susceptibility to radio. and chemotherapy. For further investigation of hypoxia.related changes, areas of certain hypoxia must be reliably detected within cancer tissues. Pimonidazole, a 2.nitroimindazole, accumulates in hypoxic tissue and can be easily visualized using immunohistochemistry. Materials and Methods: To improve detection of highly hypoxic versus normoxic areas in prostate cancer, immunoreactivity of pimonidazole and a combination of known hypoxia.related proteins was used to create computational oxygen supply maps of prostate cancer. Pimonidazole was intravenously administered before radical prostatectomy in n = 15 patients, using the da Vinci robot.assisted surgical system. Prostatectomy specimens were immediately transferred into buffered formaldehyde, fixed overnight, and completely embedded in paraffin. Pimonidazole accumulation and hypoxia.related protein expression were visualized by immunohistochemistry. Oxygen supply maps were created using the normalized information from pimonidazole and hypoxia.related proteins. Results: Based on pimonidazole staining and other hypoxia.related proteins (osteopontin, hypoxia.inducible factor 1.alpha, and glucose transporter member 1) oxygen supply maps in prostate cancer were created. Overall, oxygen supply maps consisting of information from all hypoxia.related proteins showed high correlation and mutual information to the golden standard of pimonidazole. Here, we describe an improved computer.based ex vivo model for an accurate detection of oxygen supply in human prostate cancer tissue. Conclusions: This platform can be used for precise colocalization of novel candidate hypoxia.related proteins in a representative number of prostate cancer cases, and improve issues of single marker correlations. Furthermore, this study provides a source for further in situ tests and biochemical investigations.",

keywords = "Hypoxia, pimonidazole, prostate cancer",

author = "Rupp, \{Niels J.\} and Schuffler, \{Peter J.\} and Qing Zhong and Florian Falkner and Markus Rechsteiner and Ruschoff, \{Jan H.\} and Christian Fankhauser and Matthias Drach and Remo Largo and Mathias Tremp and Cedric Poyet and Tullio Sulser and Glen Kristiansen and Holger Moch and Joachim Buhmann and Michael Muntener and Peter Wild",

note = "Publisher Copyright: {\textcopyright} 2016 Journal of Pathology Informatics | Published by Wolters Kluwer -Medknow.",

year = "2016",

month = jan,

day = "29",

doi = "10.4103/2153-3539.175376",

language = "English",

volume = "7",

journal = "Journal of Pathology Informatics",

issn = "2229-5089",

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}

Rupp, NJ, Schuffler, PJ, Zhong, Q, Falkner, F, Rechsteiner, M, Ruschoff, JH, Fankhauser, C, Drach, M, Largo, R, Tremp, M, Poyet, C, Sulser, T, Kristiansen, G, Moch, H, Buhmann, J, Muntener, M & Wild, P 2016, 'Oxygen supply maps for hypoxic microenvironment visualization in prostate cancer', Journal of Pathology Informatics, vol. 7, no. 1, 3. https://doi.org/10.4103/2153-3539.175376

TY - JOUR

T1 - Oxygen supply maps for hypoxic microenvironment visualization in prostate cancer

AU - Rupp, Niels J.

AU - Schuffler, Peter J.

AU - Zhong, Qing

AU - Falkner, Florian

AU - Rechsteiner, Markus

AU - Ruschoff, Jan H.

AU - Fankhauser, Christian

AU - Drach, Matthias

AU - Largo, Remo

AU - Tremp, Mathias

AU - Poyet, Cedric

AU - Sulser, Tullio

AU - Kristiansen, Glen

AU - Moch, Holger

AU - Buhmann, Joachim

AU - Muntener, Michael

AU - Wild, Peter

PY - 2016/1/29

Y1 - 2016/1/29

N2 - Background: Intratumoral hypoxia plays an important role with regard to tumor biology and susceptibility to radio. and chemotherapy. For further investigation of hypoxia.related changes, areas of certain hypoxia must be reliably detected within cancer tissues. Pimonidazole, a 2.nitroimindazole, accumulates in hypoxic tissue and can be easily visualized using immunohistochemistry. Materials and Methods: To improve detection of highly hypoxic versus normoxic areas in prostate cancer, immunoreactivity of pimonidazole and a combination of known hypoxia.related proteins was used to create computational oxygen supply maps of prostate cancer. Pimonidazole was intravenously administered before radical prostatectomy in n = 15 patients, using the da Vinci robot.assisted surgical system. Prostatectomy specimens were immediately transferred into buffered formaldehyde, fixed overnight, and completely embedded in paraffin. Pimonidazole accumulation and hypoxia.related protein expression were visualized by immunohistochemistry. Oxygen supply maps were created using the normalized information from pimonidazole and hypoxia.related proteins. Results: Based on pimonidazole staining and other hypoxia.related proteins (osteopontin, hypoxia.inducible factor 1.alpha, and glucose transporter member 1) oxygen supply maps in prostate cancer were created. Overall, oxygen supply maps consisting of information from all hypoxia.related proteins showed high correlation and mutual information to the golden standard of pimonidazole. Here, we describe an improved computer.based ex vivo model for an accurate detection of oxygen supply in human prostate cancer tissue. Conclusions: This platform can be used for precise colocalization of novel candidate hypoxia.related proteins in a representative number of prostate cancer cases, and improve issues of single marker correlations. Furthermore, this study provides a source for further in situ tests and biochemical investigations.

AB - Background: Intratumoral hypoxia plays an important role with regard to tumor biology and susceptibility to radio. and chemotherapy. For further investigation of hypoxia.related changes, areas of certain hypoxia must be reliably detected within cancer tissues. Pimonidazole, a 2.nitroimindazole, accumulates in hypoxic tissue and can be easily visualized using immunohistochemistry. Materials and Methods: To improve detection of highly hypoxic versus normoxic areas in prostate cancer, immunoreactivity of pimonidazole and a combination of known hypoxia.related proteins was used to create computational oxygen supply maps of prostate cancer. Pimonidazole was intravenously administered before radical prostatectomy in n = 15 patients, using the da Vinci robot.assisted surgical system. Prostatectomy specimens were immediately transferred into buffered formaldehyde, fixed overnight, and completely embedded in paraffin. Pimonidazole accumulation and hypoxia.related protein expression were visualized by immunohistochemistry. Oxygen supply maps were created using the normalized information from pimonidazole and hypoxia.related proteins. Results: Based on pimonidazole staining and other hypoxia.related proteins (osteopontin, hypoxia.inducible factor 1.alpha, and glucose transporter member 1) oxygen supply maps in prostate cancer were created. Overall, oxygen supply maps consisting of information from all hypoxia.related proteins showed high correlation and mutual information to the golden standard of pimonidazole. Here, we describe an improved computer.based ex vivo model for an accurate detection of oxygen supply in human prostate cancer tissue. Conclusions: This platform can be used for precise colocalization of novel candidate hypoxia.related proteins in a representative number of prostate cancer cases, and improve issues of single marker correlations. Furthermore, this study provides a source for further in situ tests and biochemical investigations.

KW - Hypoxia

KW - pimonidazole

KW - prostate cancer

UR - https://www.scopus.com/pages/publications/85009255604

U2 - 10.4103/2153-3539.175376

DO - 10.4103/2153-3539.175376

M3 - Article

AN - SCOPUS:85009255604

SN - 2229-5089

VL - 7

JO - Journal of Pathology Informatics

JF - Journal of Pathology Informatics

IS - 1

M1 - 3

ER -

Oxygen supply maps for hypoxic microenvironment visualization in prostate cancer

Abstract

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Keywords

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