Oximetry based on diffuse photon density wave differentials

The quantification of tissue optical properties for calculating blood saturation and hemoglobin concentration using measurements of diffuse photon density waves at some distance away from an intensity-modulated light source, generally requires the determination of the amplitude and phase of this light source. This determination may become a severe impediment for measurements performed in the clinical environment. In this work we extend a self- calibrating methodology developed for constant wave and modulation depth- phase measurements, to include amplitude and phase measurements of diffuse photon density waves. The method uses amplitude and phase changes of intensity modulated light, under the assumption of known index of refraction and invariant reduced scattering coefficient μ(s)/', to quantify the absorption coefficient μ(a) without requiring initial amplitude and phase knowledge. Quantification of the μ(a) at selected time points during a measurement can then be employed to calibrate numerical solutions of the diffusion equation and compute the μ(a) for the remaining time points of the experiment. It is shown that the method is quite insensitive to the knowledge of the exact μ(s)/' value so that an assumption on the average μ(s)/' value for the tissue measured may be employed. The sensitivity of calculating blood saturation and hemoglobin concentration, as a function of the deviation of the μ(s)/' used in the calculation versus the real μ(s)/' value is investigated using simulated data. It is also demonstrated that the saturation calculation is especially insensitive to the μ(s)/' guess. The performance of the method to quantify blood oxygen saturation and the concentrations of oxy- and deoxy-hemoglobin is examined with experimental measurements at two wavelengths on specially constructed blood model phantoms. To validate the method the measurements are monitored by a time- resolved spectrometer. The method is shown to be accurate to within ±5% in calculating blood saturation and to within ±10% in calculating hemoglobin concentration compared to the results obtained with the time-resolved spectrometer and the expected theoretical values. (C) 2000 American Association of Physicists in Medicine.