Oxidative biodegradation of tetrachloroethene in needles of Norway spruce (Picea abies L.)

Ludwig Weissflog, Gert H.J. Krüger, Sándor T. Forczek, Christian A. Lange, Karsten Kotte, Andrea Pfennigsdorff, Jana Rohlenová, Květoslava Fuksová, Hana Uhlířová, Miroslav Matucha, Peter Schröder

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Through employing [14C]-PER exposure experiments it was shown for the first time that PER taken up by drought-stressed spruce needles via the air/needle pathway is preferably degraded to trichloroacetic acid (TCA) in the chloroplasts. TCA formed by oxidative biotransformation is mineralised to CO2 and HCl via various degradation routes. HCl contributes to increased proton concentration in the chloroplast, inducing a pH shift leading to a pathophysiological effect on H+ transport from the thylakoid interior into the stroma. As a result of their high degree of dissociation and related protonation, the PER metabolites, TCA and HCl, cause a change in protein structures. In addition to this, the TCA anions created in the process may lead to destabilisation of the thylakoid membrane potential. The damage to the chloroplasts inflicted by protons and trichloroacetate ions subsequently leads to an impairment of photosynthesis, most particularly to uncoupling of photosynthetic electron transport. Since progressive aridity as consequence of the climate change observed throughout the world is predicted, a regionally variable marked enhancement of the phytotoxic risk caused by PER emission is anticipated.

Original languageEnglish
Pages (from-to)89-96
Number of pages8
JournalSouth African Journal of Botany
Volume73
Issue number1
DOIs
StatePublished - Jan 2007
Externally publishedYes

Keywords

  • Biodegradation
  • Chloroplasts
  • Drought stress
  • Norway spruce
  • Tetrachloroethene
  • Trichloroacetic acid

Fingerprint

Dive into the research topics of 'Oxidative biodegradation of tetrachloroethene in needles of Norway spruce (Picea abies L.)'. Together they form a unique fingerprint.

Cite this