Overexpression of the urokinase receptor splice variant uPAR-del4/5 in breast cancer cells affects cell adhesion and invasion in a dose-dependent manner and modulates transcription of tumor-associated genes

Bettina Grismayer; Sumito Sato; Charlotte Kopitz; Christian Ries; Susanne Soelch; Manfred Schmitt; Gustavo Baretton; Achim Kruger; Thomas Luther; Matthias Kotzsch; Viktor Magdolen

doi:10.1515/hsz-2012-0206

Overexpression of the urokinase receptor splice variant uPAR-del4/5 in breast cancer cells affects cell adhesion and invasion in a dose-dependent manner and modulates transcription of tumor-associated genes

Bettina Grismayer, Sumito Sato, Charlotte Kopitz, Christian Ries, Susanne Soelch, Manfred Schmitt, Gustavo Baretton, Achim Kruger, Thomas Luther, Matthias Kotzsch, Viktor Magdolen

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Abstract

mRNA levels of the urokinase receptor splice variant uPAR-del4/5 are associated with prognosis in breast cancer. Its overexpression in cancer cells affects tumor biologically relevant processes. In the present study, individual breast cancer cell clones displaying low vs. high uPAR-del4/5 expression were analyzed demonstrating that uPAR-del4/5 leads to reduced cell adhesion and invasion in a dose-dependent manner. Additionally, matrix metalloproteinase-9 (MMP-9) was found to be strongly upregulated in uPAR-del4/5 overexpressing compared to vector control cells. uPAR-del4/5 may thus play an important role in the regulation of the extracellular proteolytic network and, by this, influence the metastatic potential of breast cancer cells.

Original language	English
Pages (from-to)	1449-1455
Number of pages	7
Journal	Biological Chemistry
Volume	393
Issue number	12
DOIs	https://doi.org/10.1515/hsz-2012-0206
State	Published - Dec 2012

Keywords

Adhesion
Breast cancer
Differential gene expression
Invasion
MMP-9
UPAR splice variant

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Grismayer, B., Sato, S., Kopitz, C., Ries, C., Soelch, S., Schmitt, M., Baretton, G., Kruger, A., Luther, T., Kotzsch, M., & Magdolen, V. (2012). Overexpression of the urokinase receptor splice variant uPAR-del4/5 in breast cancer cells affects cell adhesion and invasion in a dose-dependent manner and modulates transcription of tumor-associated genes. Biological Chemistry, 393(12), 1449-1455. https://doi.org/10.1515/hsz-2012-0206

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title = "Overexpression of the urokinase receptor splice variant uPAR-del4/5 in breast cancer cells affects cell adhesion and invasion in a dose-dependent manner and modulates transcription of tumor-associated genes",

abstract = "mRNA levels of the urokinase receptor splice variant uPAR-del4/5 are associated with prognosis in breast cancer. Its overexpression in cancer cells affects tumor biologically relevant processes. In the present study, individual breast cancer cell clones displaying low vs. high uPAR-del4/5 expression were analyzed demonstrating that uPAR-del4/5 leads to reduced cell adhesion and invasion in a dose-dependent manner. Additionally, matrix metalloproteinase-9 (MMP-9) was found to be strongly upregulated in uPAR-del4/5 overexpressing compared to vector control cells. uPAR-del4/5 may thus play an important role in the regulation of the extracellular proteolytic network and, by this, influence the metastatic potential of breast cancer cells.",

keywords = "Adhesion, Breast cancer, Differential gene expression, Invasion, MMP-9, UPAR splice variant",

author = "Bettina Grismayer and Sumito Sato and Charlotte Kopitz and Christian Ries and Susanne Soelch and Manfred Schmitt and Gustavo Baretton and Achim Kruger and Thomas Luther and Matthias Kotzsch and Viktor Magdolen",

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month = dec,

doi = "10.1515/hsz-2012-0206",

language = "English",

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Grismayer, B, Sato, S, Kopitz, C, Ries, C, Soelch, S, Schmitt, M, Baretton, G, Kruger, A, Luther, T, Kotzsch, M & Magdolen, V 2012, 'Overexpression of the urokinase receptor splice variant uPAR-del4/5 in breast cancer cells affects cell adhesion and invasion in a dose-dependent manner and modulates transcription of tumor-associated genes', Biological Chemistry, vol. 393, no. 12, pp. 1449-1455. https://doi.org/10.1515/hsz-2012-0206

Overexpression of the urokinase receptor splice variant uPAR-del4/5 in breast cancer cells affects cell adhesion and invasion in a dose-dependent manner and modulates transcription of tumor-associated genes. / Grismayer, Bettina; Sato, Sumito; Kopitz, Charlotte et al.
In: Biological Chemistry, Vol. 393, No. 12, 12.2012, p. 1449-1455.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Overexpression of the urokinase receptor splice variant uPAR-del4/5 in breast cancer cells affects cell adhesion and invasion in a dose-dependent manner and modulates transcription of tumor-associated genes

AU - Grismayer, Bettina

AU - Sato, Sumito

AU - Kopitz, Charlotte

AU - Ries, Christian

AU - Soelch, Susanne

AU - Schmitt, Manfred

AU - Baretton, Gustavo

AU - Kruger, Achim

AU - Luther, Thomas

AU - Kotzsch, Matthias

AU - Magdolen, Viktor

PY - 2012/12

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N2 - mRNA levels of the urokinase receptor splice variant uPAR-del4/5 are associated with prognosis in breast cancer. Its overexpression in cancer cells affects tumor biologically relevant processes. In the present study, individual breast cancer cell clones displaying low vs. high uPAR-del4/5 expression were analyzed demonstrating that uPAR-del4/5 leads to reduced cell adhesion and invasion in a dose-dependent manner. Additionally, matrix metalloproteinase-9 (MMP-9) was found to be strongly upregulated in uPAR-del4/5 overexpressing compared to vector control cells. uPAR-del4/5 may thus play an important role in the regulation of the extracellular proteolytic network and, by this, influence the metastatic potential of breast cancer cells.

AB - mRNA levels of the urokinase receptor splice variant uPAR-del4/5 are associated with prognosis in breast cancer. Its overexpression in cancer cells affects tumor biologically relevant processes. In the present study, individual breast cancer cell clones displaying low vs. high uPAR-del4/5 expression were analyzed demonstrating that uPAR-del4/5 leads to reduced cell adhesion and invasion in a dose-dependent manner. Additionally, matrix metalloproteinase-9 (MMP-9) was found to be strongly upregulated in uPAR-del4/5 overexpressing compared to vector control cells. uPAR-del4/5 may thus play an important role in the regulation of the extracellular proteolytic network and, by this, influence the metastatic potential of breast cancer cells.

KW - Adhesion

KW - Breast cancer

KW - Differential gene expression

KW - Invasion

KW - MMP-9

KW - UPAR splice variant

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SN - 1431-6730

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SP - 1449

EP - 1455

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ER -

Overexpression of the urokinase receptor splice variant uPAR-del4/5 in breast cancer cells affects cell adhesion and invasion in a dose-dependent manner and modulates transcription of tumor-associated genes

Abstract

Keywords

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