@article{f2499c5d3c9d420bb0b6b4f602954500,
title = "Osteoinduction in human fat-derived stem cells by recombinant human bone morphogenetic protein-2 produced in Escherichia coli",
abstract = "Bioactive recombinant human bone morphogenetic protein-2 (rhBMP-2) was obtained using Escherichia coli pET-25b expression system: 55 mg purified rhBMP-2 were achieved per g cell dry wt, with up to 95% purity. In murine C2C12 cell line, rhBMP-2 induced an increase in the transcription of Smads and of osteogenic markers Runx2/Cbfa1 and Osterix, measured by semi-quantitative RT-PCR. Bioassays performed in human fat-derived stem cells showed an increased activity of the early osteogenic marker, alkaline phosphatase, and the absence of cytotoxicity.",
keywords = "Bone morphogenetic protein-2, Recombinant protein purification, Stem cells, Tissue engineering",
author = "Bessa, {Paulo C.} and Pedro, {A. J.} and B. Kl{\"o}sch and A. Nobre and {Van Griensven}, M. and Reis, {R. L.} and M. Casal",
note = "Funding Information: Acknowledgements The author wishes to acknowledge Rita Ara{\'u}jo for advice given in primer design, Asmita Banerjee and Daniela Dopler for help with C2C12 cells, Anja Peterbauer for providing the fat-derived mesenchymal stem cells, and Heinz Redl for critical reviewing of the manuscript. This work was supported by PhD grant SFRH/BD/17049/2004 supported by Fundac¸{\~a}o para a Ci{\^e}ncia e Tecnologia, project ElastM POCI/ CTM/57177/2004 supported by FEDER and Fundac¸{\~a}o para a Ci{\^e}ncia e Tecnologia, as well as by the European STREP Project HIPPOCRATES (NMP3-CT-2003-505758). This work was carried out under the scope of the European NoE EXPERTISSUES (NMP3-CT-2004-500283).",
year = "2008",
month = jan,
doi = "10.1007/s10529-007-9501-8",
language = "English",
volume = "30",
pages = "15--21",
journal = "Biotechnology Letters",
issn = "0141-5492",
publisher = "Springer Netherlands",
number = "1",
}