Ornithinibacillus gen. nov., with the species Ornithinibacillus bavariensis sp. nov. and Ornithinibacillus californiensis sp. nov

A Gram-positive, aerobic, rod-shaped, motile, endospore-forming bacterium was isolated from pasteurized milk from Bavaria, Germany. 16S rRNA gene sequence similarities indicated that strain WSBC 24001^T was most closely related to Virgibacillus species (95.3-96.1%), Oceanobacillus species (95.6-95.7%), Bacillus firmus IAM 12464^T (95.5%) and Bacillus niacini IFO 15566^T (95.2%). However, strain WSBC 24001^T showed the highest level of sequence similarity to an unnamed strain, MB-9^T (97.6%), which was isolated from coastal surface sediments in California. Hence, this strain was included in our study. The genomic DNA G+C contents of strains WSBC 24001^T and MB-9^T were 36.4 mol and 40.8 mol%, respectively. The major respiratory quinone of both strains was menaquinone MK-7 and the peptidoglycan type was A4β (L-Orn←-D-Asp). The polar lipid profiles of these strains contained a predominance of diphosphatidylglycerol and moderate to minor amounts of phosphatidylglycerol, an unknown phospholipid and an unknown aminophospholipid. However, strain WSBC 24001^T could be distinguished from strain MB-9^T by the presence of an unknown lipid. The fatty acid profiles of the two strains comprised mainly iso- and anteiso-branched acids, but showed some significant quantitative differences in the amounts of certain acids. The DNA-DNA relatedness value (15.5%) clearly demonstrated that strains WSBC 24001^T and MB-9^T are representatives of two different species. On the basis of their phylogenetic position and morphological, physiological and chemotaxonomic properties, a novel genus is proposed, Ornithinibacillus gen. nov., with two novel species, the type species Ornithinibacillus bavariensis sp. nov. (type strain WSBC 24001^T=DSM 15681^T=CCM 7096^T) and Ornithinibacillus californiensis sp. nov. (type strain MB-9^T =DSM 16628^T=CCM 7237^T).